當(dāng)前位置 ： Millipore >>> Millipore/03-112 | RIPAb+ QKI-5/03-112/10 assays10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent
Millipore/03-112 | RIPAb+ QKI-5/03-112/10 assays10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent
  • Millipore/03-112 | RIPAb+ QKI-5/03-112/10 assays10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent

Millipore/03-112 | RIPAb+ QKI-5/03-112/10 assays10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent

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貨號: 03-112
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    • Description
      CatalogueNumber03-112
      BrandFamilyUpstate
      TradeName
      • RIPAb+
      • Upstate
      DescriptionRIPAb+QKI-5
      OverviewRIPAb+antibodiesareevaluatedusingtheRNABindingProteinImmunoprecipitation(RIP)assay.EachRIPAb+antibodysetincludesanegativecontrolantibodytoensurespecificityoftheRIPreactionandisverifiedfortheco-immunoprecipitationofRNAassociatedspecificallywiththeimmunoprecipitatedRNAbindingproteinofinterest.Whereappropriate,theRIPAb+setalsoincludesquantitativeRT-PCRcontrolprimers(RIPPrimers)toBIOLOGicallyvalidateyourIPresultsbysuccessfullyco-precipitatingthespecificRNAtargets,suchasmessengerRNAs.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateRIPprotocolswhenusingtheantibodyintheirexperimentalcontext.Ifatargetspecificassayisnotprovided,theRIPAb+kitisvalidatedusinganautomatedmicrofluidics-basedassaybyenrichmentofdetectableRNAovercontrolimmunoprecipitation.
      AlternateNames
      • QuakingProtein
      BackgroundInformationQKIisanRNA-bindingproteinthatplaysacentralroleinmyelinization.Actsbyregulatingpre-mRNAsplicing,mRNAexport,mRNAstABIlityandproteintranslation.Itisregulatedbyalternativesplicing.
      ProductInformation
      FormatPurified
      Control
      • IncludesnegativecontrolrabbitIgGantibodyandcontrolprimersspecificforhumanMAGI1.
      PresentationAnti-QKI-5(RabbitPolyclonal).Onevialcontaining50μLofrabbitserum.Storeat-20°C.

      NormalRabbitIgG.Onevialcontaining125μgRabbitIgGin125μLstoragebuffercontaining0.05%sodiumazide.Storeat-20°C.

      RIPPrimers,MAGI1.Onevialcontaining75μLof5μMofeachprimerspecificforhumanMAGI1.Storeat-20°C.
      FOR:TATTCCAAACCACCCTCCAA
      REV:CAACTCAGAAGGGTTTCGTGT
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      KeyApplications
      • RNABindingProteinImmunoprecipitation(RIP)
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunoprecipitationfromRIPlysate:
      Representativelotdata.
      RIPlysatefromHeLacells(2X106cellequivalentsperIP)wassubjectedtoimmunoprecipitationusing0.5μLofeitheranormalrabbitIgGor0.5μLofAnti-QKI-5antibody(Pleaseseefigures).

      AutomatedMicrofluidics-basedElectrophoreticRNASeparationandAnalysis:
      RIPLysatepreparedfromNTera-2cells(2X107cellequivalentsperIP)weresubjectedtoimmunoprecipitationusing5μLofeitheranormalrabbitIgGor5μLofAnti-QKI-5antibodyandtheMagnaRIP?RNA-BindingProteinImmunoprecipitationKit(Cat.#17-700).
      SuccessfulimmunoprecipitationofQKI-5-associatedRNAwasverifiedbyautomatedmicrofluidics-basedelectrophoreticRNAseparationandanalysis.
      PleaserefertotheMagnaRIP?(Cat.#17-700)orEZ-MagnaRIP?(Cat.#17-701)protocolforexperimentaldetails.

      Electropherogramsweregeneratedbyplottingfluorescenceintensitiesversusmigrationtimes(Pleaseseefigures).
      Avirtualgelviewwascreatedfromthisdata(Pleaseseefigures).

      WesternBlotAnalysis:1:500-1:1,000dilutionofapreviouslotdetectedQKI-5inwesternblot.
      BiologicalInformation
      ImmunogenSyntheticpeptidecorrespondingtomouseQKI-5.
      HostRabbit
      SpecificityRecognizesQKI-5(QuakingProtein).
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryQKIbelongstoafamilyofRNA-bindingproteinsthathaveanHNRNPK(MIM600712)homology(KH)domainembeddedina200-aminoacidregioncalledtheGSGdomain.OthermembersofthisfamilyincludeSAM68(KHDRBS1;MIM602489)andSF1(MIM601516)(ChenandRichard,1998[PubMed9671495]).[suppliedbyOMIM]
      GeneSymbol
      • QKI
      • DKFZp586I0923
      • Hqk
      • QK3
      • QK
      • HKQ
      • HqkI
      PurificationMethodSerum
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q96PU8#RNA-bindingproteinthatplaysacentralroleinmyelinization.Bindstothe5"-NACUAAY-N(1,20)-UAAY-3"RNAcoresequence.Actsbyregulatingpre-mRNAsplicing,mRNAexport,mRNAstabilityandproteintranslation.RequiredtoprotectandpromotestabilityofmRNAssuchasMBPandCDKN1Bwhichpromotesoligodendrocytedifferentiation.ParticipatesinmRNAtransportbyregulatingthenuclearexportofMBPmRNA.AlsoinvolvedinregulationofmRNAsplicingofMAGpre-mRNA.Actsasatranslationalrepressor(Bysimilarity).
      SIZE:341aminoacids;37671Da
      SUBUNIT:Homodimer.DoesnotrequireRNAtohomodimerize.AbletoheterodimerizewithBICC1(Bysimilarity).
      SUBCELLULARLOCATION:Nucleus(Bysimilarity).Cytoplasm(Bysimilarity).
      TISSUESPECIFICITY:Expressedinthefrontalcortexofbrain.
      PTM:MethylatedbyPRMT1(Bysimilarity).&TyrosinephosphorylatedatitsC-terminus,probablybyFYN.PhosphorylationleadstodecreasedmRNA-bindingaffinity,affectingtransportand/orstabilizationofMBPmRNA(Bysimilarity).
      DISEASE:SwissProt:Q96PU8#Mayplayaroleingeneticsusceptibilitytoschizophrenia.QKIlevelsofsomeisoformssuchasisoform6,arefrequentlydown-regulatedinschizophrenicpatients.&DeletionoftheQKIgenemayplayaroleinastrocytictumors.
      SIMILARITY:SwissProt:Q96PU8##Contains1KHdomain.
      MolecularWeight~40kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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