Immunogen | Phospho-specificpeptidecorrespondingtoresiduessurroundingSer9ofhumanGSK3β. |
Epitope | PhosphorylatedSer9 |
Clone | EPR2286Y |
Host | Rabbit |
Specificity | TheantibodyonlydetectsGSK3βphosphorylatedonSer9. |
Isotype | IgG |
SpeciesReactivity | |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Glycogensynthasekinase-3(GSK3)isaproline-directedserine-threoninekinasethatwasinitiallyidentifiedasaphosphorylatingandinactivatingglycogensynthase(seeGYS1,MIM138570).Twoisoforms,alpha(GSK3A;MIM606784)andbeta,showahighdegreeofaminoacidhomology(StambolicandWoodgett,1994[PubMed7980435]).GSK3Bisinvolvedinenergymetabolism,neuronalcelldevelopment,andbodypatternformation(Plyteetal.,1992[PubMed1333807]). |
GeneSymbol | |
Modifications | |
PurificationMethod | Unpurified |
UniProtNumber | |
UniProtSummary | FUNCTION:ParticipatesintheWntsignalingpathway.Implicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN.PhosphorylatesJUNatsitesproximaltoitsDNA-bindingdomain,therebyreducingitsaffinityforDNA.PhosphorylatesMUC1inbreastcancercells,anddecreasestheinteractionofMUC1withCTNNB1/beta-catenin.Ref.5 CATALYTICACTIVITY:ATP+[tauprotein]=ADP+[tauprotein]phosphate. ENZYMEREGULATION:InhibitedwhenphosphorylatedbyAKT1. SUBUNITSTRUCTURE:MonomerBysimilarity.InteractswithCABYR,MUC1,NINandPRUNE. TISSUESPECIFICITY:Expressedintestis,thymus,prostateandovaryandweaklyexpressedinlung,brainandkidney. PTM:PhosphorylatedbyAKT1andILK1. SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.GSK-3subfamily. Contains1proteinkinasedomain. |
MolecularWeight | ~47kDa |