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Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL
  • Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL

Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL

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貨號: 04-349
品牌: Millipore
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    • Description
      CatalogueNumber04-349
      DescriptionAnti-MAPKinase2/Erk2Antibody,cloneE460,rabbitmonoclonal
      AlternateNames
      • mitogen-activatedproteinkinase1
      • Extracellularsignal-regulatedkinase2
      • Mitogen-activatedproteinkinase2
      • MAPkinase1
      • MAPkinase2
      • MAPkinaseisoformp42
      • proteintyrosinekinaseERK2
      BackgroundInformationExtracellularsignalregulatedkinase2(ERK2)alsoknownasmaturationormitogenactivatedproteinkinase(MAPK)isinvolvedineukaryoticsignaltransduction(1).Activatedbyadualphosphorylationonthreonineandtyrosineresiduebythedual-specificitykinaseMKK1/2,ERK2p42translocatetothenucleusafterstimulation(2).Oncephosphorylated,ERK2canformdimerswithphosphorylatedandunphosphorylatedERK2partners.ItisalsoabletophosphorylatemultiplesubstratessuchasEGFR,c-PLA2,Elk1/TCFandc-Myc(3-5).
      ProductInformation
      FormatUnpurified
      Control
      • A431celllysate
      PresentationRabbitMonoclonalinbuffercontainingglycerol,BSA,andsodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingcap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20oC.Avoidfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunoprecipitation
      • FlowCytometry
      • Immunohistochemistry(Paraffin)
      ApplicationNotesImmunohistochemistryAnalysis:A1:250-500dilutionfromarepresentativelotwasusedinhumanbreastcarcinomatissue.
      ImmunocytochemistryAnalysis:A1:100-250dilutionfromarepresentativelotwasusedinIC.
      ImmunoprecipitationAnalysis:A1:80dilutionfromarepresentativelotwasusedinIP.
      FlowCytometryAnalysis:A1:50dilutionfromarepresentativelotwasusedinFC.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtotheC-terminusofhumanMAPKinase2/Erk2.
      EpitopeC-terminus
      CloneE460
      HostRabbit
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • ERK2
      • PRKM1
      • ERK
      • MAPK2
      • p41mapk
      • ERK-2
      • ERT1
      • PRKM2
      • MAPK2
      • p42-MAPK
      • EC2.7.11.24
      • OTTHUMP00000028754
      • OTTHUMP00000174492
      • P42MAPK
      • p38
      • p40
      • p41
      • MAPK1
      • EC2.7.11
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:Serine/threoninekinasewhichactsasanessentialcomponentoftheMAPkinasesignaltransductionpathway.MAPK1/ERK2andMAPK3/ERK1arethe2MAPKswhichplayanimportantroleintheMAPK/ERKcascade.TheyparticipatealsoinasignalingcascadeinitiatedbyactivatedKITandKITLG/SCF.Dependingonthecellularcontext,theMAPK/ERKcascademediatesdiversebiologicalfunctionssuchascellgrowth,adhesion,survivalanddifferentiationthroughtheregulationoftranscription,translation,cytoskeletalrearrangements.ThetheMAPK/ERKcascadeplaysalsoaroleininitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactors.About160substrateshavealreadybeendiscoveredforERKs.Manyofthesesubstratesarelocalizedinthenucleus,andseemtoparticipateintheregulationoftranscriptionuponstimulation.However,othersubstratesarefoundinthecytosolaswellasinothercellularorganelles,andthoseareresponsIBLeforprocessessuchastranslation,mitosisandapoptosis.Moreover,theMAPK/ERKcascadeisalsoinvolvedintheregulationoftheendosomaldynamics,includinglysosomeprocessingandendosomecyclingthroughtheperinuclearrecyclingcompartment(PNRC);aswellasinthefragmentationoftheGolgiapparatusduringmitosis.Thesubstratesincludetranscriptionfactors(suchasATF2,BCL6,ELK1,ERF,FOS,HSF4orSPZ1),cytoskeletalelements(suchasCANX,CTTN,GJA1,MAP2,MAPT,PXN,SORBS3orSTMN1),regulatorsofapoptosis(suchasBAD,BTG2,CASP9,DAPK1,IER3,MCL1orPPARG),regulatorsoftranslation(suchasEIF4EBP1)andavarietyofothersignaling-relatedmolecules(likeARHGEF2,FRS2orGRB10).Proteinkinases(suchasRAF1,RPS6KA1/RSK1,RPS6KA3/RSK2,RPS6KA2/RSK3,RPS6KA6/RSK4,SYK,MKNK1/MNK1,MKNK2/MNK2,RPS6KA5/MSK1,RPS6KA4/MSK2,MAPKAPK3orMAPKAPK5)andphosphatases(suchasDUSP1,DUSP4,DUSP6orDUSP16)areothersubstrateswhichenablethepropagationtheMAPK/ERKsignaltoadditionnalcytosolicandnucleartargets,therebyextendingthespecificityofthecascade.
      Actsasatranscriptionalrepressor.Bindstoa[GC]AAA[GC]consensussequence.Represstheexpressionofinterferongamma-inducedgenes.SeemstobindtothepromoterofCCL5,DMP1,IFIH1,IFITM1,IRF7,IRF9,LAMP3,OAS1,OAS2,OAS3andSTAT1.Transcriptionalactivityisindependentofkinaseactivity.
      CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein.
      COFACTOR:MagnesiumBysimilarity.
      ENZYMEREGULATION:PhosphorylatedbyMAP2K1/MEK1andMAP2K2/MEK2onThr-185andTyr-187inresponsetoexternalstimulilikeinsulinorNGF.Bothphosphorylationsarerequiredforactivity.Thisphosphorylationcausesdramaticconformationalchanges,whichenablefullactivationandinteractionofMAPK1/ERK2withitssubstrates.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DephospphorylatedandinactivatedbyDUSP3,DUSP6andDUSP9.Inactivatedbypyrimidylpyrroleinhibitors.
      SUBUNITSTRUCTURE:Bindsbothupstreamactivatorsanddownstreamsubstratesinmultimolecularcomplexes.BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithADAM15,ARHGEF2,ARRB2,DAPK1(viadeathdomain),HSF4,IER3,IPO7,DUSP6,NISCH,SGK1,andisoform1ofNEK2.Interacts(phosphorylatedform)withCAV2("Tyr-19"-phosphorylatedform);theinteraction,promotedbyinsulin,leadstonuclearlocationandMAPK1activation.InteractswithMORG1,PEA15andMKNK2Bysimilarity.MKNK2isoform1bindingpreventsfromdephosphorylationandinactivationBysimilarity.
      SUBCELLULARLOCATION:Nucleus.Cytoplasm?Cytoskeleton?centrosome.Cytoplasm.Note:PEA15-bindingandphosphorylatedDAPK1promoteitscytoplasmicretention.PhosphorylationatSer-246andSer-248aswellasautophosphorylationatThr-190promotenuclearlocalization.
      DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases.
      PTM:PhosphorylateduponKITandFLT3signalingBysimilarity.DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme.UndergoesregulatoryphosphorylationonadditionalresiduessuchasSer-246andSer-248inthekinaseinsertdomain(KID)Thesephosphorylations,whichareprobablymediatedbymorethanonekinase,areimportantforbindingofMAPK1/ERK2toimportin-7(IPO7)anditsnucleartranslocation.Inaddition,autophosphorylationofThr-190wasshowntoaffectthesubcellularlocalizationofMAPK1/ERK2aswell.Ligand-activatedALKinducestyrosinephosphorylation.DephosphorylatedbyPTPRJatTyr-187.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DUSP3andDUSP6dephosphorylatespecificallyMAPK1/ERK2andMAPK3/ERK1whereasDUSP9dephosphorylatesabroaderrangeofMAPKs.
      SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.
      Contains1proteinkinasedomain.
      SEQUENCECAUTION:ThesequenceCAA77753.1differsfromthatshown.Reason:Erroneousinitiation.TranslationN-terminallyextended.
      MolecularWeight~42kDaobserved
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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