Immunogen | KLH-conjugated,syntheticpeptidecontainingthesequence(GKGGA[acetyl-K]RHRKC,correspondingtothesequencesurroundingacetylatedlysine16ofhumanhistoneH4. |
Epitope | AcetylLys16 |
Clone | 4E10.2 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Mouse |
Specificity | RecognizeshistoneH4acetylatedonLys16,Mr11kDa. |
Isotype | IgG1κ |
SpeciesReactivity | |
SpeciesReactivityNote | Basedonsequencehomology,broadspeciescross-reactivityisexpected. |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.[providedbyRefSeq].
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Modifications | |
PurificationMethod | ProteinGPurified |
UniProtNumber | |
UniProtSummary | FUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
Subunitstructure:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
Subcellularlocation:Nucleus.
Post-translationalmodification:AcetylationatLys-6,Lys-9,Lys-13andLys-17occursincodingregionsofthegenomebutnotinheterochromatin.
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MolecularWeight | ~11kDa |