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Millipore/05-665 | Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7/05-665/100 µg
  • Millipore/05-665 | Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7/05-665/100 µg

Millipore/05-665 | Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7/05-665/100 µg

價格: ￥5052.00 市場價: 8420.00
貨號: 05-665
品牌: Millipore
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    • Description
      CatalogueNumber05-665
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-Active-β-Catenin(Anti-ABC)Antibody,clone8E7
      BackgroundInformationCateninsareproteinsfoundincomplexeswithcadherincelladhesionmoleculesofanimalcells.Thefirsttwocateninsthatwereidentifiedbecameknownasα-cateninandβ-catenin.α-catenincanbindtoβ-cateninandcanalsobindactin.β-cateninbindsthecytoplasmicdomainofsomecadherins.
      Whenβ-cateninwassequenceditwasfoundtobeamemberofthearmADIllofamilyofproteins.Theseproteinshavemultiplecopiesoftheso-calledarmadillorepeatdomainwhichisspecializedforprotein-proteinbinding.Anincreaseinβ-cateninproductionhasbeennotedinthosepeoplewhohaveBasalCellCarcinomaandleadstotheincreaseinproliferationofrelatedtumors.Whenβ-cateninisnotassociatedwithcadherinsandα-catenin,itcaninteractwithotherproteinssuchasCateninBetaInteractingProtein1(ICAT)andadenomatosispolyposiscoli(APC).
      Recentevidencesuggeststhatβ-cateninplaysanimportantroleinvariousaspectsofliverBIOLOGyincludingliverdevelopment(bothembryonicandpostnatal),liverregenerationfollowingpartialhepatectomy.HGF-inducedhepatpomegaly,liverzonation,andpathogenesisoflivercancer.
      ProductInformation
      FormatPurified
      Control
      • PositiveControlIncluded:Non-StimA431CellLysate(12-301).Othercontrols:HeLawholecelllysateorMCF7wholecelllysate
      PresentationPurifiedmousemonoclonalIgG1kinbuffercontaining0.1MTris-Glycine,pH7.4,15mMNaCland0.05%sodiumazide
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat2-8°Cfromdateofshipment.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.
      Applications
      ApplicationAnti-Active-β-Catenin(anti-ABC)Antibody,clone8E7isawellcharacterizedMouseMonoclonalAntibody.ThishighlypublishedmAbalsoknownasAnti-Cateninbeta-1readilydetectsbeta-Catenin&hasbeenvalidatedinFC,ICC,IHC,IHC(P)&WB.
      KeyApplications
      • FlowCytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesImmunocytochemistry:Thisantibodywasusedinimmunocytochemistryandshowedpositivestainingonthemembraneandcytosol.
      Thisantibodyhasalsobeenreportedbyanindependentlaboratorytoshowpositiveimmunostainingforbeta-catenininLiCl-treated293Tcellsfixedwithmethanol(Staal,FrankJ.T.,2002).

      FlowCytometry:Thisantibodywasusedinflowcytometryatanoptimal1μg/mLconcentration.

      Immunohistochemistry:Thisantibodywasusedinimmunohistochemistryonacolorectalcarcinomatissuearrayata1:300dilution.
      Thisantibodyhasalsobeenreportedbyanindependentlaboratorytodetectbeta-catenininmouseembryosections(VanNoort,M.,2002).
      BiologicalInformation
      ImmunogenFusionproteincorrespondingtoresidues1-100ofhumanβ-cateninwithac-terminalhistidinetag
      EpitopeTheepitopecorrespondstoaminoacidresidues36-44(HSGATTTAP)ofhumanβ-catenin.
      Clone8E7
      HostMouse
      SpecificityRecognizesactiveformofβ-catenin,dephosphorylatedonSer37orThr41.
      IsotypeIgG1κ
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      SpeciesReactivityNoteTheantibodyeptiopeis100%conservedinzebrafish.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryBeta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactinCytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM]
      GeneSymbol
      • CTNNB1
      • CTNNB
      • FLJ25606
      • Beta-catenin
      • PRO2286
      • FLJ37923
      • DKFZp686D02253
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P35222#InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway.
      SIZE:781aminoacids;85497Da
      SUBUNIT:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.ThestABIlizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andDUPLIN.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1binding(Bysimilarity).InteractswiththePDZdomainofTAX1BP3;thisinteractioninhibitsthetranscriptionalactivityofCTNNB1(Bysimilarity).InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2.InteractswithSLC30A9(Bysimilarity).
      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2promotesnucleartranslocation.
      TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon.
      PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33.&EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding.&UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1X(Probable).Itsubiquitinationleadstoitssubsequentproteasomaldegradation.
      DISEASE:"SwissProt:P35222#ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife.&DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor.&DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren.&AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1."
      SIMILARITY:SwissProt:P35222##Belongstothebeta-cateninfamily.&Contains12ARMrepeats.
      MolecularWeight92kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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