Immunogen | Inordertoproducebroadspectrumpolyclonalphosphotyrosineantibodies,rabbitswereimmunizedwiththreephosphorylatedimmunogens:(1)phosphotyrosinecovalentlylinkedtoKLH;(2)thec-Srccarboxylterminalregulatoryphosphopeptide(TS-T-E-P-Q-pY-Q-P-G-E-N-L;Catalog#12-218)covalentlylinkedtoKLH,and;(3)aphosphopeptideassociatedwithhightyrosinekinaseactivityinhumanlymphocytes(R-R-L-I-E-D-A-E-pY-A-A-R-G;Catalog#12-217)covalentlylinkedtoKLH.BothofthephosphopeptidehaptensserveasstrongsubstratesfortyrosinephosphatasesandarepartofthetwocolorimetrictyrosinephosphatasekitsprovidedbyUpstate,Inc.(Catalog#17-125,17-126). |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | Theimmunoreactivityoftheantibodyistotallyinhibitedbytheuseof100mMphenylphosphate,aphosphotyrosineanalog.Thephosphotyrosineantibodyispurifiedbyimmunoaffinitychromatographyusingeitheradualphospho-peptidegeloraBSA-phosphotyrosinegel.Allofthephosphotyrosineimmunoreactivitypresentintheantiseraisimmunoadsorbedwhethertheantibodyispurifiedbyeithergelindicatingthattheantibodyisnotsequence-specificbutspecificforphosphotyrosineresidues. |
SpeciesReactivity | |
SpeciesReactivityNote | Human.PredictedtoreactwithAllbasedon100%sequencehomology. |
AntibodyType | PolyclonalAntibody |
Modifications | |
PurificationMethod | AffinityPurfied |
MolecularWeight | Varies |