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Millipore/07-1398 | Anti-phospho-Akt (Thr308) Antibody/07-1398/100 µL
  • Millipore/07-1398 | Anti-phospho-Akt (Thr308) Antibody/07-1398/100 µL

Millipore/07-1398 | Anti-phospho-Akt (Thr308) Antibody/07-1398/100 µL

價格: ￥3444.00 市場價: 5740.00
貨號: 07-1398
品牌: Millipore
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    • Description
      CatalogueNumber07-1398
      DescriptionAnti-phospho-Akt(Thr308)Antibody
      AlternateNames
      • ProteinkinaseB
      • RAC-alphaserine/threonine-proteinkinase
      • murinethymomaviral(v-akt)oncogenehomolog-1
      • racproteinkinasealpha
      • v-aktmurinethymomaviraloncogenehomolog1
      BackgroundInformationAkt/PKBisaSer/ThrkinaseandamajorknowneffecterofthePI3Kinasepathway.ItisinvolvedinmultiplesignalingpathwaysthatrelatetomanyBIOLOGicalprocessesincludingmetabolism,apoptosis,cellcyclecontrol,angiogenesis,differentiation,andcellgrowthandproliferation.Inmammals,threeisoformsofAkt(Akt1/PKCα,Akt2/PKBβ,andAkt3/PKBγ)exists.Theyexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Akt1isthepredominantisoformthatisinmosttissuesandisthoughttohaveadominantroleingrowth,survival,embryonicdevelopment,andADIpocytedifferentiation.Akt2iscorrelatedwiththeregulationofglucosehomeostasisandisthepredominantisoformexpressedininsulin-responsivetissues.Akt3isabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalPleckstrinHomology(PH)domainthatprovidesalipid-bindingmodule,acentralcatalyticdomaincontainingThr308,andaC-terminalhydrophobicmotifcontainingSer473.TheactivationofAKTisdependentonadualregulatorymechanismthatrequiresbothitstranslocationtotheplasmamembraneanddualphosphorylationonThr308andSer473byPDK1andtheTORC2complex,respectively.
      ProductInformation
      FormatAffinityPurified
      PresentationAntigenaffinitypurifiedrabbitserumin0.1MTris-Glycine(pH7.4),150mMNaCl,with0.05%NaN3.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.
      Applications
      ApplicationThisAnti-phospho-Akt(Thr308)AntibodyisvalidatedforuseinIH(P)forthedetectionofphospho-Akt(Thr308).
      KeyApplications
      • Immunohistochemistry(Paraffin)
      ApplicationsNotRecommended
      • WesternBlotting
      ApplicationNotesImmunohistochemistryonly

      WesternBlotAnalysis:
      NotrecommendedforWBasitgivesmultiplebands.Pleasereferto05-802R
      BiologicalInformation
      ImmunogenKLH-conjugatedsyntheticpeptidecontainingtheThr308sequenceandsurroundingaminoacidsofhuman,mouse,rat,bovineandchickenAkt1.HumanandmouseAkt2shares11/12aminoacidsandratAkt3shares10/12aminoacidswiththepeptideimmunogensequence.
      EpitopeThr308(CatalyticDomain)
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityDetectsAktonlywhenphosphorylatedonThr308.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Xenopus
      • Canine
      SpeciesReactivityNoteMousetested.ExpectedtoreactwithHuman,Rat,Chicken,Dog,Xenopus,andCowbasedon100%immunogensequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.[providedbyRefSeq].

      TranscriptVariant:Thisvariant(3)lacksthe5"exon,buthasaupstreamalternate5"exon,ascomparedtovariant1.Variants1and3encodethesameprotein.
      GeneSymbol
      • AKT
      • C-AKT
      • EC2.7.11.1
      • MGC99656
      • PKB
      • PKB-ALPHA
      • RAC
      • RAC-ALPHA
      • RAC-PK-alpha
      Modifications
      • Phosphorylation
      PurificationMethodAntigenaffinitypurifiedagainstboththephosphorylatedandthenon-phosphorylatedpeptidesofAkt(Thr308).
      UniProtNumber
      UniProtSummaryFUNCTION:Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase.Ref.4Ref.8Ref.12Ref.13Ref.16Ref.20

      CatalyticactivityATP+aprotein=ADP+aphosphoprotein.Ref.4Ref.20Ref.1

      ENZYMEREGULATION:Threespecificsites,oneinthekinasedomain(Thr-308)andthetwootheronesintheC-terminalregulatoryregion(Ser-473andTyr-474),needtobephosphorylatedforitsfullactivation.Ref.8Ref.6

      SUBUNITSTRUCTURE:InteractswithAGAP2isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDNandTHEM4.InteractswithAKTIP.Interacts(viaPHdomain)withMTCP1,TCL1AANDTCL1B.InteractswithCDKN1B;theinteractionphosphorylatesCDKN1Bpromoting14-3-3bindingandcell-cycleprogression.Ref.13Ref.16Ref.20Ref.9Ref.10Ref.11Ref.15Ref.17Ref.18Ref.19

      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cellmembrane.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).NucleartranslocationisenhancedbyinteractionwithTCL1A.Ref.11

      TISSUESPECIFICITY:Inallhumancelltypessofaranalyzed.Ref.4

      DOMAIN:BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane.

      TheAGC-kinaseC-terminalmediatesinteractionwithTHEM4.

      PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithAGAP2isoform2(PIKE-A).Ser-473phosphorylationisenhancedinfocalcorticaldysplasiaswithTaylor-typeballooncells.

      SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.

      Contains1AGC-kinaseC-terminaldomain.

      Contains1PHdomain.

      Contains1proteinkinasedomain.

      MolecularWeight60kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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