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Millipore/07-1413 | Anti-GSK-3b Antibody, a.a. 335-349/07-1413/50 µg
  • Millipore/07-1413 | Anti-GSK-3b Antibody, a.a. 335-349/07-1413/50 µg

Millipore/07-1413 | Anti-GSK-3b Antibody, a.a. 335-349/07-1413/50 µg

價格: ¥3696.00 市場價: 6160.00

貨號: 07-1413
品牌: Millipore
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    • Description
      CatalogueNumber07-1413
      ReplacesAB8687
      DescriptionAnti-GSK-3bAntibody,a.a.335-349
      BackgroundInformationGSK3(GlycogenSynthaseKinase3)differsfrommostserine/threoninekinasesinthatitisactiveintheabsenceoftheactionofsignalingpathways.Twoisoformsexist,GSK3αandGSK3β.ThefunctionofGSK3istophosphorylateGlycogenSynthaseandtherebyinactivateit.InsulinactionstimulatesthePI3Kinasepathway,resultinginAktactivation,whichphosphorylatesandinactivatesGSK3.GlycogenSynthaseisthenrapidlydephosphorylatedandactivated.OtherGSK3substratesincludeJun(oninhibitorysites),andeIF2B.PhosphorylationofTaubyGSK3mayrelatetodevelopmentofAlzheimer"sdisease.Phosphorylationstate-specificantibodiesdirectedagainsttheAktsite(Ser21)onGSK3aresuitableforsurrogateassaysoftheactivationstateofthepathway.
      ProductInformation
      FormatAffinityPurified
      Control
      • C2C12CellLysate,Helacells,ratormousebrain
      PresentationPurifiedpolyclonalIgGantibodyissuppliedinPBS,pH7.2,50%glyceroland0.09%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStableat–20oCforuptooneyearfromdateofreceipt.Productmaybestoredatfor2-4weeksat2o-8oC.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationThisAntiI-GSK-3bAntibody,a.a.335-349isvalidatedforuseinWB,IP,IH(P)forthedetectionofAntiI-GSK-3b.
      KeyApplications
      • WesternBlotting
      • Immunoprecipitation
      • Immunohistochemistry(Paraffin)
      ApplicationNotesImmunoprecipitation:12.5μg/mLdilutionofapreviouslotwasuedinimmunoprecipitationonratbraintissue;immunoprecipitationswithtissueextractsotherthanbrainmayrequirehigherantibodyconcentrations.
      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      Immunohistochemistry(paraffin):
      Representativetestingfromapreviouslot.
      OptimalStainingofGSK3βPolyclonalAntibody:HeLaCells
      BIOLOGicalInformation
      ImmunogenRatGSK3betasyntheticpeptide(aa.335-349),conjugatedtoKLH.Thissequenceis100%identicaltohumanGSK3beta.
      Epitopea.a.335-349
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityThisantibodydetectsastrong47kDaprotein,correspondingtotheexpectedmolecularmassofGSK3beta.
      IsotypeIgG
      SpeciesReactivity
      • Rat
      • Human
      • Mouse
      • Bovine
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryGlycogensynthasekinase-3(GSK3)isaproline-directedserine-threoninekinasethatwasinitiallyidentifiedasaphosphorylatingandnactivatingglycogensynthase.Twoisoforms,alpha(GSK3A;MIM606784)andbeta,showahighdegreeofaminoacidhomology(StambolicandWoodgett,1994[PubMed7980435]).GSK3Bisinvolvedinenergymetabolism,neuronalcelldevelopment,andbodypatternformation(Plyteetal.,1992[PubMed1333807]).[suppliedbyOMIM].
      GeneSymbol
      • GSK3B
      • EC2.7.11.26
      PurificationMethodProteinAaffinitypurified
      UniProtNumber
      UniProtSummaryFUNCTION:ParticipatesintheWntsignalingpathway.Implicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN.PhosphorylatesJUNatsitesproximaltoitsDNA-bindingdomain,therebyreducingitsaffinityforDNA.PhosphorylatesMUC1inbreastcancercells,anddecreasestheinteractionofMUC1withCTNNB1/beta-catenin.
      CATALYTICACTIVITY:ATP+[tauprotein]=ADP+[tauprotein]phosphate.
      ENZYMEREGULATION:InhibitedwhenphosphorylatedbyAKT1.
      SUBUNIT:Monomer(Bysimilarity).InteractswithCABYR,MUC1,NINandPRUNE.
      ALTERNATIVEPRODUCTS:2namedisoforms[FASTA]producedbyalternativesplicing.
      MolecularWeightThisantibodydetectsastrong47kDaprotein.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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