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Millipore/07-1570 | Anti-monomethyl Histone H4 (Lys20) Antibody/07-1570/100 µg
  • Millipore/07-1570 | Anti-monomethyl Histone H4 (Lys20) Antibody/07-1570/100 µg

Millipore/07-1570 | Anti-monomethyl Histone H4 (Lys20) Antibody/07-1570/100 µg

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貨號: 07-1570
品牌: Millipore
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    • Description
      CatalogueNumber07-1570
      DescriptionAnti-monomethylHistoneH4(Lys20)Antibody
      AlternateNames
      • H4K20me1
      • HistoneH4(monomethylK20)
      • H4histonefamily,memberA
      • histone1
      • H4a
      • histonecluster1
      • H4a
      BackgroundInformationHistonesarehighlyconservedproteinsthatserveasthestructuralscaffoldfortheorganizationofnuclearDNAintochromatin.HistonesmodificationsregulateDNAtranscription,repair,recombination,andreplication.Themostcommonlystudiedmodificationsareacetylation,phosphorylation,methylation,andubiquitination.Thesemodificationscanalterlocalchromatinarchitecture,orrecruittrans-actingfactorsthatrecognizespecifichistonemodifications(the"histonecode"hypothesis).MethylationofhistoneH4atlysine20hasbeenimplicatedintranscriptionalactivation,genesilencing,heterochromatinformation,mitosis,andDNArepair.HistoneH4isprogressivelymethylatedatLys20duringtheG(2),M,andG(1)phasesofthecellcycle,andmethylationofH4Lys20isaMarkerforheterochromatin.Inmammals,thefemaleXchromosomeiscoatedbytheXistRNA,whichiscriticalforsilencing,andismarkedmainlybymethylationofH3Lys9,H3Lys27andH4Lys20.
      ProductInformation
      FormatAffinityPurified
      Control
      • HeLacellacidextracts.
      PresentationPurifiedfromserumbyaffinitychromatography.Suppliedat1mg/mlin0.1MTris-Glycine(pH7.4),15mMNaCl,0.05%NaN3.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationAnti-monomethylHistoneH4(Lys20)AntibodyisarabbitpolyclonalantibodyfordetectionofmonomethylHistoneH4(Lys20)alsoknownasH4K20me1,HistoneH4(monomethylK20),H4histonefamilymemberA&hasbeenvalidatedinWB.
      KeyApplications
      • WesternBlotting
      • DotBlot
      • ChromatinImmunoprecipitation(ChIP)
      • PeptideInhibitionAssay
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(2X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing4μgofeitheranegativecontrolantibodyorAnti-Monomethyl-HistoneH4
      (Lys20)antibodyandtheMagnaChIPAKit(Cat.#17-610).Successfulimmunoprecipitationofmonomethyl-histoneH4(Lys20)-associatedDNAfragmentswasverifiedbyqPCRusingGAPDHcodingregionChIPPrimersversusControlPrimerscorrespondingtotheGAPDHpromoter(Pleaseseefigures).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatin,withimmunoprecipitatedDNAfromnegativecontrolantibodyshownas(-)andmonomethyl-histoneH4(Lys20)shownas(+).
      PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

      DotBlotAnalysis:
      AbsuranceHistoneH3AntibodySpecificityArray(Cat.No.16-667)andAbsuranceHistoneH2A,H2B,H4AntibodySpecificityArray(Cat.No.16-665),whichcontainhistonepeptideswithvariousmodificationswereprobedwithCat.No.07-1570Anti-monomethylHistoneH4(Lys20)at1:1000dilution.ProteinswerevisualizedusingaDonkeyanti-rabbitIgGconjugatedtoHRPandachemiluminescencedetectionsystem.

      PeptideBlockingAssay:
      40μgofhistoneH4peptidecontainingmonomethyllysine20abolisheddetectionofhistoneH4byanti-monomethyl-HistoneH4(Lys20)inimmunoblotanalysisofacidextractsofHeLacells
      BIOLOGicalInformation
      ImmunogenKLH-conjugated,linearsyntheticpeptidecorrespondingtoaminoacids15-24
      (AKRHR[me1K]VLRD-C)ofhumanHistoneH4.
      EpitopeMonomethylLys20
      HostRabbit
      SpecificityRecognizesmonomethylatedHistoneH4
      (Lys20),Mr11kDa.
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteHuman.Basedonsequencehomology,broadspeciescross-reactivityisexpected
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.[providedbyRefSeq]
      GeneSymbol
      • H4/A
      • H4/B
      • H4/C
      • H4/D
      • H4/E
      • H4/G
      • H4/H
      • H4/I
      • H4/J
      • H4/K
      • H4/M
      • H4/N
      • H4/O
      • H4/a
      • H4F2
      • H4FA
      • H4FB
      • H4FC
      • H4FD
      • H4FE
      • H4FG
      • H4FH
      • H4FI
      • H4FJ
      • H4FK
      • H4FM
      • H4FN
      • H4FO
      • HIST2H4
      Modifications
      • Methylation
      PurificationMethodAffinityChromatography
      UniProtNumber
      UniProtSummaryFUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA

      SUBCELLULARLOCATION:Nucleus.
      PTM:AcetylationatLys-6,Lys-9,Lys-13andLys-17occursincodingregionsofthegenomebutnotinheterochromatin.
      PTM:CitrullinationatArg-4byPADI4impairsmethylation.
      PTM:MonomethylationatArg-4byPRMT1favorsacetylationatLys-9andLys-13.DemethylationisperformedbyJMJD6.
      PTM:Monomethylated,dimethylatedortrimethylatedatLys-21.MonomethylationisperformedbySET8.TrimethylationisperformedbySUV420H1andSUV420H2andinducesgenesilencing.
      PTM:UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirradiation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      PTM:Sumoylated,whichisassociatedwithtranscriptionalrepression.
      SIMILARITY:BelongstothehistoneH4family.
      MolecularWeight~11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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