GoldBio/CSF1 (M-CSF), Human/2 μg/1120-09-2

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

RecombinantHuman Colony Stimulating Factor 1 (macrophage)MacrophageColony Stimulating Factor; M-CSFCSF1

Colony Stimulating Factor 1 (CSF1), previously called Macrophage Colony Stimulating Factor (M-CSF), is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone), exerts paracrine effects on osteoclasts and can interact with CSF1R. CSF1 is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for CSF1 gene. Although forms may vary, all of them contain the N-terminal 150 amino acid portion that is necessary and sufficient for interaction with the receptor. The first 223 amino acid of mature human CSF1 shares 88%, 86%, 81% and 74% sequence identity with corresponding regions of dog, cow, mouse and rat CSF1, respectively. Human CSF1 is active in the mouse, but mouse CSF1 is reported to be species-specific.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence:EEVSEYCSHMIGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQLQELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQGHERQSEGS

MW: ~36.8 kDa (2x 158 amino acids)

Purity: >95% by SDS-PAGEand HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0×10⁶ IU/mg.

Accession Number: P09603

HGNC ID:2432

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using sterile distilled water to a concentration of 0.1mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stocksolutions should be aliquoted and stored at -80°C. Further dilutions should bemade in appropriate buffered solutions.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!