Boc-protected Cyanine5 hydrazide reactive dye for the labeling of aldehydes and ketones.
Boc- (tert-butoxycarbonyl) protective group can be removed with trifluoroacetic acid, or other strong acid. Reactive hydrazide is formed, which can then couple with various carbonyl groups yielding Cyanine5-labeled molecules.
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General properties
Appearance: | dark blue powder |
Molecular weight: | 633.26 |
Molecular formula: | C37H49ClN4O3 |
Solubility: | good in dichloromethane, polar organic solvents (DMF, DMSO), alcohols, low in water |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
Spectral properties
Excitation maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 250000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |