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Millipore/17-327 | H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)/17-327/1 kit192 assays
  • Millipore/17-327 | H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)/17-327/1 kit192 assays

Millipore/17-327 | H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)/17-327/1 kit192 assays

價(jià)格: ￥8388.00 市場(chǎng)價(jià): 13980.00
貨號(hào): 17-327
品牌: Millipore
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    • Description
      CatalogueNumber17-327
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionH2A.XPhosphorylationAssayKit(ChemiluminescenceDetection)
      OverviewPhosphorylationofthehistonevariantH2A.XisarapidandsensitiveresponsetodoublestrandDNAbreaks.TheH2A.XPhosphorylationAssayKit(ChemiluminescenceDetection)isacellbasedassayformattedformicroplate-baseddetectionoflevelsofphosphorylatedhistoneH2A.X.
      ProductInformation
      Components
      • Anti-phospho-HistoneH2A.X(Ser139),cloneJBW301(Cat.#05-636)
      • TBS,20X(Cat.#20-190)
      • 20%Tween-20(v/v)
      • 10%BSAinTBS(BlockingBuffer)
      • LumiGLOSubstrateReagentA
      • LumiGLOSubstrateReagentB
      • GoatAnti-MouseIgG,HRPconjugate
      DetectionmethodChemiluminescence
      StorageandShippingInformation
      Applications
      ApplicationTheH2A.XPhosphorylation,ChemiluminescenceDetectionAssayKitisacell-basedELISAformattedforchemiluminescentdetectionofrelativelevelsofphosphorylatedH2A.Xinmicroplatecellscultures.
      KeyApplications
      • ELISA
      BIOLOGicalInformation
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneencodesamemberofthehistoneH2Afamily,andgeneratestwotranscriptsthroughtheuseoftheconservedstem-loopterminationmotif,andthepolyAadditionmotif.
      GeneSymbol
      • H2AFX
      • H2A/X
      • H2A.X
      • H2AX
      • H2a/x
      Modifications
      • Phosphorylation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P16104#VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Requiredforcheckpoint-mediatedarrestofcellcycleprogressioninresponsetolowdosesofionizingrADIationandforefficientrepairofDNAdoublestrandbreaks(DSBs)specificallywhenmodifiedbyC-terminalphosphorylation.
      SIZE:143aminoacids;15145Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.InteractswithnumerousproteinsrequiredforDNAdamagesignalingandrepairwhenphosphorylatedonSer-140.TheseincludeMDC1,TP53BP1,BRCA1andtheMRNcomplex,composedofMRE11A,RAD50,andNBN.InteractionwiththeMRNcomplexismediatedatleastinpartbyNBN.AlsointeractswithDHX9/NDHIIwhenphosphorylatedonSer-140.
      SUBCELLULARLOCATION:Nucleus.DEVELOPMENTALSTAGE:SynthesizedinG1aswellasinS-phase.
      DOMAIN:SwissProt:P16104The[ST]-QmotifconstitutesarecognitionsequenceforkinasesfromthePI3/PI4-kinasefamily.
      PTM:PhosphorylatedonSer-140(toformgamma-H2AFX)inresponsetoDNAdoublestrandbreaks(DSBs)generatedbyexogenousgenotoxicagentsandbystalledreplicationforks,andmayalsooccurduringmeioticrecombinationeventsandimmunoglobulinclassswitchinginlymphocytes.PhosphorylationcanextenduptoseveralthousandnucleosomesfromtheactualsiteoftheDSBandmaymarkthesurroundingchromatinforrecruitmentofproteinsrequiredforDNAdamagesignalingandrepair.Widespreadphosphorylationmayalsoservetoamplifythedamagesignaloraidrepairofpersistentlesions.PhosphorylationofSer-140inresponsetoionizingradiationismediatedbybothATMandPRKDCwhiledefectsinDNAreplicationinduceSer-140phosphorylationsubsequenttoactivationofATRandPRKDC.DephosphorylationofSer-140byPP2AisrequiredforDNADSBrepair.Inmeiosis,Ser-140phosphorylationmayoccuratsynaptonemalcomplexesduringleptoteneasanATM-dependentresponsetotheformationofprogrammedDSBsbySPO11.Ser-140phosphorylationmaysubsequentlyoccursatunsynapsedregionsofbothautosomesandtheXYbivalentduringzygotene,downstreamofATRandBRCA1activation.Ser-140phosphorylationmayalsoberequiredfortranscriptionalrepressionofunsynapsedchromatinandmeioticsexchromosomeinactivation(MSCI),wherebytheXandYchromosomescondenseinpachytenetoformtheheterochromaticXY-body.Duringimmunoglobulinclassswitchrecombinationinlymphocytes,Ser-140phosphorylationmayoccuratsitesofDNA-recombinationsubsequenttoactivationoftheactivation-inducedcytidinedeaminaseAICDA.&MonoubiquitinationofLys-120byRING1andRNF2/RING2complexgivesaspecifictagforepigenetictranscriptionalrepression(Bysimilarity).
      SIMILARITY:BelongstothehistoneH2Afamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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