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Millipore/17-469 | Met STAR ELISA Kit/17-469/96 assays
  • Millipore/17-469 | Met STAR ELISA Kit/17-469/96 assays

Millipore/17-469 | Met STAR ELISA Kit/17-469/96 assays

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貨號: 17-469
品牌: Millipore
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    • Description
      CatalogueNumber17-469
      BrandFamilyUpstate
      TradeName
      • STAR
      • Upstate
      DescriptionMetSTARELISAKit
      BackgroundInformationTestPrinciple
      TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheMetplateiscoatedwithaspecificmousemonoclonalanti-Metcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingMetantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-MetantibodytodetectthecapturedMetontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve.

      MetBackground
      MetisatyrosinekinasereceptorforHepatocyteGrowthFactor(HGF),thoughttostimulatemultiplecellularprocessesincludingcellproliferation,differentiation,cellmigrationandtumOrigenesis.ChronicstimulationofMetoncancercellsisthoughttoplayaroleinmetastasis.
      MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes
      2.Plateshaker(optional)
      3.Pipettors&tipscapableofaccuratelymeasuring1-1000μL
      4.GraduatedSEROlogicalpipettes
      5.96-wellmicrotiterPlateReaderwith450nmfilter
      6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
      7.Microfugetubesforstandardandsampledilutions
      8.Mechanicalvortex
      9.1litercontainer
      10.Distilledordeionizedwater
      ProductInformation
      Components
      • 1.CapturePlatepre-coatedwithanti-humanMetantibody:(PartNo.17-469A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
      • 2.Anti-Metdetectionantibody:(PartNo.17-469B)Onebottle(11mL)ofanti-Metdetectionantibodycontainingsodiumazide,readytouse.
      • 3.ELISADiluent:(PartNo.17-469C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
      • 4.25XELISAWashBuffer:(PartNo.17-469D)Onebottle(50mL)of25XELISAWashBuffer.
      • 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-469E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosol.
      • 6.HRPDiluent:(PartNo.17-469F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol.
      • 7.TMBSolution:(PartNo.17-469G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
      • 8.StopSolution:(PartNo.17-469H)Onebottle(25mL)ofstopsolution,readytouse.
      • 9.MetStandard:(PartNo.17-469I)TwovialsofMetstandard,lyophilized.
      • 10.PlateCovers:Twoplatecovers.
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditions1yearat4°C
      Applications
      ApplicationTheMetcolorimetricSTARELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtoquantifyspecificlevelsofsignalingtargetsindenaturedcellextracts.
      KeyApplications
      • ELISA
      BIOLOGicalInformation
      SpeciesReactivity
      • Human
      AnalytesAvailable
      • Met
      EntrezGeneNumber
      EntrezGeneSummaryTheproto-oncogeneMETproductisthehepatocytegrowthfactorreceptorandencodestyrosine-kinaseactivity.Theprimarysinglechainprecursorproteinispost-translationallycleavedtoproducethealphaandbetasubunits,whicharedisulfidelinkedtoformthematurereceptor.VariousmutationsintheMETgeneareassociatedwithpapillaryrenalcarcinoma.
      GeneSymbol
      • MET
      • HGFR
      • AUTS9
      • c-Met
      • RCCP2
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P08581#Receptorforhepatocytegrowthfactorandscatterfactor.Hasatyrosine-proteinkinaseactivity.
      SIZE:1390aminoacids;155527Da
      SUBUNIT:Heterodimerformedofanalphachain(50kDa)andabetachain(145kDa)whicharedisulfidelinked.BindsPLXNB1andGRB2.InteractswithSPSB1,SPSB2andSPSB4(Bysimilarity).InteractswithINPP5D/SHIP1.WhenphosphorylatedatTyr-1356,interactswithINPPL1/SHIP2.InteractswithRANBP9andRANBP10.
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      DISEASE:SwissProt:P08581#ActivationofMETafterrearrangementwiththeTPRgeneproducesanoncogenicprotein.&DefectsinMETmaybeassociatedwithgastriccancer.&DefectsinMETareacauseofhepatocellularcarcinoma(HCC)[MIM:114550].&DefectsinMETareacauseofhereditarypapillaryrenalcarcinoma(HPRC)[MIM:605074];alsoknownaspapillaryrenalcellcarcinoma2(RCCP2).HPRCisaformofinheritedkidneycancercharacterizedbyapredispositiontodevelopmultiple,bilateralpapillaryrenaltumors.Thepatternofinheritanceisconsistentwithautosomaldominanttransmissionwithreducedpenetrance.&GeneticvariationsinMETmaybeassociatedwithsusceptibilitytoautismtype1B(AUTS1B)[MIM:608636].Autismisaneurodevelopmentaldisordercharacterizedbydisturbanceinlanguage,perceptionandsocialization.Thedisorderisclassicallydefinedbyatriadoflimitedorabsentverbalcommunication,alackofreciprocalsocialinteractionorresponsiveness,andrestricted,stereotypical,andritualizedpatternsofinterestsandbehavior.
      SIMILARITY:SwissProt:P08581##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.&Contains3IPT/TIGdomains.&Contains1proteinkinasedomain.&Contains1Semadomain.
      PhysicochemicalInformation
      Sensitivity
      • Sensitivity:0.4ng/mL.
        RangeofDetection:0.4to25ng/mL
      Dimensions
      MaterialsInformation
      MaterialsInformation
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