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Millipore/AB144P-200UL | Anti-Choline Acetyltransferase Antibody/AB144P-200UL/200 µL
  • Millipore/AB144P-200UL | Anti-Choline Acetyltransferase Antibody/AB144P-200UL/200 µL

Millipore/AB144P-200UL | Anti-Choline Acetyltransferase Antibody/AB144P-200UL/200 µL

價(jià)格: ¥3444.00 市場價(jià): 5740.00

貨號: AB144P-200UL
品牌: Millipore
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    • Description
      CatalogueNumberAB144P-200UL
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CholineAcetyltransferaseAntibody
      AlternateNames
      • Cholineacetylase
      • acetylCoA:cholineO-acetyltransferase
      • cholineacetyltransferase
      BackgroundInformationAcetylcholine(ACh)isacommonneuro-transmitterformotoneurons,preganglionicautonomicneurons,postganglionicpara-sympatheticneurons,avarietyofbrainregionsandsomeemergingneuron-likestemcells.ThemetabolismofAchisrelativelysimple,involvingonlytwoenzymes:cholineacetyltransferase(ChAT)forsynthesisandacetylcholinesterase(AChE)fordegradation.Further,acetylcholinehaslittlefunctioninneuronsotherthanneurotransmissionandseemstobeneuronspecific.ItseemsthatonlycholinergicneuronshavesignificantamountsofChATmakinganti-cholinacetyltransferaseausefulspecificMarker.
      ProductInformation
      FormatAffinityPurified
      Control
      • Rathypothalamustissue.
      PresentationPurifiedgoatpolyclonalinbuffercontainingPBScontaining5mg/mLBSAand0.2%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cinundilutedaliquotsfromdateofreceipt.
      Forshorttermstorage(3-4weeks)maintainat+2-8°C.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisAnti-CholineAcetyltransferase(ChAT)AntibodyisvalidatedforuseinIC,IH,WBforthedetectionofCholineAcetyltransferase.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:
      Apreviouslotofthisantibodywasusedinimmunohistochemistryat1:100dilution.Seeattachedprotocol.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenHumanplacentalenzyme.
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostGoat
      SpecificityCholineacetyltransferasefoundincholinergicneuronsinbrainandcentralnervoussystems.
      SpeciesReactivity
      • Mouse
      • Rat
      • Human
      • Chicken
      • Avian
      • GuineaPig
      • Opossum
      SpeciesReactivityNoteRat,mouse,monkey,guineapig,chicken,opossum,avianandhuman(westernblot).
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesanenzymewhichcatalyzesthebiosynthesisoftheneurotransmitteracetylcholine.Thisgeneproductisacharacteristicfeatureofcholinergicneurons,andchangesintheseneuronsmayexplainsomeofthesymptomsofAlzheimerdisease.Mutationsinthisgeneareassociatedwithcongenitalmyasthenicsyndromeassociatedwithepisodicapnea.Multipletranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene,andsomeofthesevariantshavebeenshowntoencodemorethanoneisoform.[providedbyRefSeq].

      TranscriptVariant:Thisvariant(M)containsalternate5"exonM,usesanupstreamstartcodon,andencodesisoform2.
      GeneSymbol
      • CHAT
      • ChAT
      • CHOACTase
      • CMS1A
      • CMS1A2
      PurificationMethodImmunoAffinityPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P28329#CatalyzesthereversIBLesynthesisofacetylcholine(ACh)fromacetylCoAandcholineatcholinergicsynapses.
      SIZE:748aminoacids;82568Da
      DISEASE:SwissProt:P28329#DefectsinCHATarethecauseoffamilialinfantilemyastheniagravis2(FIMG2)[MIM:254210,254200];alsoknownasCMS-EA.FIMG2patientshavemyasthenicsymptomssincebirthorearlyinfancy,negativetestsforanti-AChRantibodies,andabruptepisodiccriseswithincreasedweakness,bulbarparalysis,andapneaprecipitatedbyundueexertion,fever,orexcitement.Inheritanceisautosomalrecessive.
      SIMILARITY:SwissProt:P28329##Belongstothecarnitine/cholineacetyltransferasefamily.
      MolecularWeight70kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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