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Millipore/AB1530 | Anti-Peripherin Antibody/AB1530/100 µL
  • Millipore/AB1530 | Anti-Peripherin Antibody/AB1530/100 µL

Millipore/AB1530 | Anti-Peripherin Antibody/AB1530/100 µL

價格: ¥4188.00 市場價: 6980.00

貨號: AB1530
品牌: Millipore
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    • Description
      CatalogueNumberAB1530
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-PeripherinAntibody
      BackgroundInformationPeripherinisa56-58kDatypeIIIintermediatefilamentprotein(Portieretal.1983),expressedinmotor,sensoryandsympatheticsystemneuronsaswellasneuroendocrineskincarcinomasandcertainmelanocytictumors.Itisoftenusedanindicatorofentericganglioncelldensityinnormalanddiseased(e.g.Hirschsprung"sdisease)states.AbnormalinclusionscontainingperipherinhavealsobeenreportedinALS.Duringnormal,lateneurogenesisperipherinappearsaftertheexpressionofnestin,vimentin,alpha-internexinandneurofilamentsandisinvolvedinthedifferentiationofneurons,namelyoftheperipheralnervoussystem,andalsoprobablyinaxonalregeneration.Peripherinisnotrelatedtoperipherin-RDS,aphotoreceptorproteinassociatedwithretinaldegenerationandblindness.
      ProductInformation
      FormatSerum
      Control
      • Ratsensoryneurons,ratspinalcordhomogenateandperipheralnervehomogenate.
      PresentationRabbitpolyclonalantisera,nopreservatives.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cinundilutedaliquotsfromdateofreceipt.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisAnti-PeripherinAntibodyisvalidatedforuseinIH,IH(P),WBforthedetectionofPeripherin.
      KeyApplications
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesAdditionalResearchApplications

      Immunohistochemistry:
      Apreviouslotofthisantibodywasusedat1:100-1:200dilution.Itissuggestedthattheantibodybeusedonfrozensectionsfixedinacetoneat-20°C.AB1530willworkontissuefixedforonehourorlessinfresh4%paraformaldehyde.Ithasbeenreportedthatthisantibodycanbeusedonparaffinembeddedtissuesections.SeeCell&TissueResearch(1997)288:11-23&EuropeanJ.Dermatology(1998)8:339-342.

      ElectronMicroscopy:
      ApreviouslotofthisantibodywasusedonElectronMicroscopy.

      Optimalworkingdilutionsandprotocolsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenElectrophoreticallypuretrp-E-peripherinfusionprotein[Dev.BrainRes.(1990)57:235-248],containingallbutthe4Nterminalaminoacidsofratperipherin.FusionproteinpurifiedfrombacterialinclusionbodiesbyDEAE-cellulosechromatographyin6MureafollowedbypreparativeSDS-PAGE.
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityRecognizesPeripherin.Theantibodystainsa~57kDabandcleanlyandspecificallyanddoesnotstainvimentin,GFAP,alpha-internexinoranyoftheneurofilamentsubunits.Strongstainingonrat,mouse,human,pigandcowperipherin.Doesnotstainchicken,quailorothermoredistantlyrelatedspecieswhichappeartolackperipherin.
      SpeciesReactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Pig
      SpeciesReactivityNoteStrongstainingonratandhuman.Cross-reactswithmouse,pig,andbovine.Otherspeciesnottested.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesacytoskeletalproteinfoundinneuronsoftheperipheralnervoussystem.TheencodedproteinisatypeIIIintermediatefilamentproteinwithhomologytoothercytoskeletalproteinssuchasdesmin,andisadifferentproteinthattheperipherinfoundinphotoreceptors.Mutationsinthisgenehavebeenassociatedwithsusceptibilitytoamyotrophiclateralsclerosis.
      GeneSymbol
      • PRPH
      • Peripherin
      • PRPH1
      • peripherin
      • NEF4
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P41219#Class-IIIneuronalintermediatefilamentprotein.
      SIZE:470aminoacids;53651Da
      SIMILARITY:SwissProt:P41219##Belongstotheintermediatefilamentfamily.
      MolecularWeight56-58kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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