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Millipore/AB3623 | Anti-Caspase 3 Antibody, active (cleaved) form/AB3623/50 µg
  • Millipore/AB3623 | Anti-Caspase 3 Antibody, active (cleaved) form/AB3623/50 µg

Millipore/AB3623 | Anti-Caspase 3 Antibody, active (cleaved) form/AB3623/50 µg

價格: ￥4068.00 市場價: 6780.00
貨號: AB3623
品牌: Millipore
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    • Description
      CatalogueNumberAB3623
      Replaces04-440;04-1088
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Caspase3Antibody,active(cleaved)form
      AlternateNames
      • Apopain
      • YamaProtein
      • CASP3
      • SCA-1
      • procaspase3
      BackgroundInformationCaspase-3(CPP32,Apopain)isthemostextensivelystudiedapoptoticprotein.Caspase-3issynthesizedasaninactiveproenzyme(32kDa)thatisprocessedincellsundergoingapoptosisbyself-proteolysisand/orcleavagebyanotherupstreamprotease.Theprocessedformofcaspase-3consistsoflarge(17kDa)andsmall(12kDa)subunitswhichassociatetoformanactiveenzyme.Theactivecaspase-3proteolyticallycleavesandactivatesothercaspases,aswellasrelevanttargetsinthecellssuchasPARPandDFF.
      ProductInformation
      FormatAffinityPurified
      Control
      • Tonsilandappendixtissue.JurkatcellsinducedforapoptosiswithrecombinantsolubleTRAILandstaurosporinetreatedmouse3T3celllysatesSkin/Tumor,Embryo.
      PresentationPurifiedrabbitpolyclonalinbuffercontainingPBS,containing50%glycerol,1%BSAand0.02%thimerosal.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      Applications
      ApplicationAnti-Caspase3Antibody,active(cleaved)formdetectslevelofCaspase3andhasbeenpublishedandvalidatedforuseinImmunofluorescence(IF),Immunohistochemistry(IHC),andWesternBlot(WB).
      KeyApplications
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesWesternBlotAnalysis:
      1:100-1:200(0.5-4μg/mL)dilutionofactiveapoptoticcellsweusedtodetectCaspase-3.
      Notewhenusingtissue,levelsofactivecaspase3canbelowdetectablelevels.UseCC119(humancaspase3)incubated30minuteswith1mMATP(foractivation)asapositivecontrolonwesterns.Alternatively,neuronalculturepreparationsincubatedwithstaurosporine(0.5μM,10-18hr)typicallygiveastrongcaspase3signal.

      Immunohistochemistry:
      1:10dilution(10-20μg/mL),AB3623hasreactedsuccessfullyin4%PFAfixed,paraffinembeddedtissuesmousetissues.

      Immunohistochemistry(paraffin):
      OptimalStainingofCaspase-3MonoclonalAntibody:MouseSmallIntestine
      BIOLOGicalInformation
      ImmunogenCleavagespecificsyntheticpeptidesequence(proprietary).
      Epitopeactive(cleaved)form
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityAB3623detectsonlythecleavedp17fragmentanddoesnotdetecttheprecursorformincellsundergoingapoptosis.
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      SpeciesReactivityNoteHuman,Mouse,Rat.Reactivitywithotherspecieshasnotbeendetermined.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.
      GeneSymbol
      • CASP3
      • CPP-32
      • Yama
      • CASP-3
      • CPP32B
      • SCA-1
      • CPP32
      • EC3.4.22.56[Contains:Caspase-3subunitp17Caspase-3subunitp12].
      PurificationMethodImmunoAffinityPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin.
      SIZE:277aminoacids;31608Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem.
      PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa.
      SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family.
      MolecularWeight17kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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