ApplicationNotes | Westernblot:1:1,000-1:10,000usingECL.Recognizesabandof200-220kDa.
Immunohistochemistry:1:1,000-1:10,000onfrozenorparaffinembeddedtissuesections.Suggestedfixativeis4%paraformaldehyde.
Immunocytochemistry:1:1,000-1:10,000oncellsintissueculturefixedforoneminutein3.7%formalinandoneminutein-20°Cmethanol.
Optimalworkingdilutionsmustbedeterminedbytheenduser. |