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Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays
  • Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays

Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays

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貨號: APT116
品牌: Millipore
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    • Description
      CatalogueNumberAPT116
      BrandFamilyChemicon®
      TradeName
      • Apo-BrdU
      • Chemicon
      DescriptionApo-BRDUIHCKit
      OverviewTheCHEMICONAPO-BRDU-IHC?KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyimmunohistochemistry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding:positiveandnegativecontrolslidesforassessingreagentperformance;reactionandblockingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),biotin-labeledanti-BrdUantibodyforlabelingDNAstrandbreaks,HRP-streptavidinconjugate,DAN,H2O2/Ureatabletsforcolorgeneration,andmethylgreensolutionforcounterstainingthecells.
      MaterialsRequiredbutNotDelivered1.Xylene-100%

      2.Ethanol-100%,90%,80%,70%v/vwithdH2O

      3.Methanol-100%

      4.HydrogenPeroxide-30%

      5.PBSorTris-bufferedsaline(1XTBS,20mMTrispH7.6,140mMNaCl)

      6.10mMTris,pH8.0

      7.1mMMgSO4in1XTBS(foruseingeneratingpositivecontrol)

      8.DNaseI(foruseingeneratingpositivecontrol)

      9.1-4%FormaldehydeinPBS,pH7.4

      10.DistilledWater

      11.Coplinjarswithslideholders

      12.Beakerforrinsingslides

      13.Slidehumidifyingchamber

      14.Glasscoverslips

      15.Mountingmedia

      16.Microscope

      17.Micro-pipettorwithdisposabletips

      18.Microcentrifugetubes

      19.Parafilm?(RegisteredtrademarkofAmericanNationalCanCompany)

      20.Absorbentwipes

      21.Crushedice
      ProductInformation
      Components
      • Positive/NegativeControlSlides,Naturalbox
      • Note:2slides,eachcontainingamixturepositiveandnegativecontrolcells.
      • BlockingBuffer,Whitecap
      • H2O2/UreaTablets,Ambervial
      • ProteinaseK,Pinkcap
      • DABTablets,Ambervial
      • TdTEnzyme,Yellowcap
      • Br-Dutp,Violetcap
      • 200XStreptavidin-HRPConjugate,Blackcap
      • 5XReactionBuffer,Greencap
      • Biotin-labeledBrdUmAb,Orangecap
      • MethylGreensolution,Naturalcap,
      • TheAPO-BRDU-IHC?Kitisshippedinonecontainerandshouldbestoredat-20°Cuponarrival.
      • Note:Afterthekitisdefrostedbeforefirstuse,boththe5XReactionBufferandtheBiotin-labeledanti-BrdUantibodyshouldbestoredat2-8°C.Also,theMethylGreensolutionshouldbestoredatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.
      • Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess50slidesincluding2positive/negativecontrolslides.Thecontrolcellsarederivedfromahumanpromyelociticleukemiacellline(HL60)andincubatedwith0.5mg/mLactinomycinDfor19hourstoinduceapoptosis.
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsPrecautionsandWarnings:

      1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures.

      2.Afterinitialdefrosting,storethe5XReactionBufferandBiotin-labeledanti-BrdUantibodyat2-8oC,andtheMethylGreensolutionatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.

      3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution.

      4.The5XReactionbufferandBr-dUTPsolutioncontaincacodylicacidasabuffer.TheBiotin-labeledantibodycontains0.05%sodiumazideasapreservative.TheDABtabletscontaindiaminobenzidine.Thesematerialsareharmfulifswallowed.Avoidskincontactwithanyofthesecomponents;washimmediatelywithwater.

      5.Uponwarmingthecomponents,centrifugethetubesfor30secondstoforcealltheliquidtothebottomofthetube.

      6.Gloves,labcoatandprotectiveeyewearshouldbewornwhilestaining.
      Applications
      ApplicationTheAPO-BRDU-IHCKitisatwocolorstainingmethodforlabelingDNAbreaks&totalcellularDNAtodetectapoptoticcellsbyIHC.
      KeyApplications
      • Immunohistochemistry
      BIOLOGicalInformation
      SpeciesReactivity
      • All
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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