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Millipore/APT139 | Caspase 9 Colorimetric Activity Assay Kit, LEHD/APT139/25 assays
  • Millipore/APT139 | Caspase 9 Colorimetric Activity Assay Kit, LEHD/APT139/25 assays

Millipore/APT139 | Caspase 9 Colorimetric Activity Assay Kit, LEHD/APT139/25 assays

價(jià)格: ¥3300.00 市場(chǎng)價(jià): 5500.00

貨號(hào): APT139
品牌: Millipore
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    • Description
      CatalogueNumberAPT139
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionCaspase9ColorimetricActivityAssayKit,LEHD
      OverviewActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.Chemicon"sCaspase-9ColorimetricActivityAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizetheLEHD.Theassayisbasedonspectophotometricdetectionofthechromophorep-nitroaniline(pNA)aftercleavagefromthelabeledsubstrateLEHD-pNA.ThefreepNAcanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat405nm.ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-9activity.
      AlternateNames
      • Mch-6
      • ICE-LAP6
      • Apaf-3
      MaterialsRequiredbutNotDelivered·Microcentrifugeand1.5mLMicrocentrifugetubes

      ·37°CWaterbathorIncubator

      ·SpectrophotometerorMicroplateReader

      ·96wellMicrotiterPlate
      ProductInformation
      Components
      • 5XCellLysisBuffer(PartNo.90065):5mL
      • 5XAssayBuffer(PartNo.90066):10mL
      • Caspase-9Substrate(Ac-LEHD-pNA)(PartNo.90083):250μLof3mg/mLsolution
      • Caspase-9Inhibitor(Ac-LEHD-CHO)(PartNo.90087):50μLof100μM(0.054mg/mL)inDMSO
      • pNAStandard(PartNo.90085):250μLof10mMinDMSO
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStorekitmaterialsat-20°Cuptotheirexpirationdate.

      SpecialPrecautions:

      ·Afterthawingreagents,useimmediatelyoraliquotandfreezeat-20°Cforlongerstorage.Avoidrepeatedfreeze/thawcycles.

      ·TheCaspase-9substrateandpNAstandardareespeciallylightsensitive.Maintainthesereagentsinamberorcoveredcontainers.
      Applications
      ApplicationCaspase-9ColorimetricActivityAssayKitsprovideasimple&convenientmeansforassayingtheactivityofcaspasesthatrecognizetheLEHD.
      KeyApplications
      • ActivityAssay
      BIOLOGicalInformation
      SpeciesReactivity
      • Mammals
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisproteinisprocessedbycaspaseAPAF1;thisstepisthoughttobeoneoftheearliestinthecaspaseactivationcascade.Alternativesplicingresultsintwotranscriptvariantswhichencodedifferentisoforms.
      GeneSymbol
      • CASP9
      • CASP-9
      • ICE-LAP6
      • APAF3
      • MCH6
      • CASPASE-9c
      • OTTHUMP00000044594
      • APAF-3
      • EC3.4.22.62[Contains:Caspase-9subunitp35
      • Caspase-9subunitp10].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P55211#Isoform2lacksactivityisandominant-negativeinhibitorofcaspase-9.
      SIZE:416aminoacids;46281Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya35kDa(p35)anda10kDa(p10)subunit.Caspase-9andAPAF1bindtoeachotherviatheirrespectiveNH2-terminalCED-3homologousdomainsinthepresenceofcytochromeCandATP.InteractswiththeinhibitorsBIRC2,BIRC4,BIRC5andBIRC7.
      TISSUESPECIFICITY:Ubiquitous,withhighestexpressionintheheart,moderateexpressioninliver,skeletalmuscle,andpancreas.Lowlevelsinallothertissues.
      PTM:CleavagesatAsp-315bygranzymeBandatAsp-330bycaspase-3generatethetwoactivesubunits.Caspase-8and-10canalsobeinvolvedintheseprocessingevents.
      SIMILARITY:SwissProt:P55211##BelongstothepeptidaseC14family.&Contains1CARDdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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