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Millipore/APT171 | Caspase 8 Colorimetric Activity Assay Kit, IETD/APT171/100 assays
  • Millipore/APT171 | Caspase 8 Colorimetric Activity Assay Kit, IETD/APT171/100 assays

Millipore/APT171 | Caspase 8 Colorimetric Activity Assay Kit, IETD/APT171/100 assays

價(jià)格: ￥6108.00 市場價(jià): 10180.00
貨號(hào): APT171
品牌: Millipore
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    • Description
      CatalogueNumberAPT171
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionCaspase8ColorimetricActivityAssayKit,IETD
      OverviewActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.Chemicon"sCaspase-8ColorimetricActivityAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceIETD.Theassayisbasedonspectophotometricdetectionofthechromophorep-nitroaniline(pNA)aftercleavagefromthelabeledsubstrateIETD-pNA.ThefreepNAcanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat405nm.ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-8activity.
      AlternateNames
      • FLICE
      MaterialsRequiredbutNotDelivered·Microcentrifugeand1.5mLMicrocentrifugetubes

      ·37°CWaterbathorIncubator

      ·SpectrophotometerorMicroplateReader

      ·96wellMicrotiterPlate
      ProductInformation
      Components
      • 5XCellLysisBuffer(PartNo.90065):5mL
      • 5XAssayBuffer(PartNo.90066):10mL
      • Caspase-8Substrate(Ac-IETD-pNA)(PartNo.90082):1mLof2.5mg/mLsolution
      • Caspase-8Inhibitor(Ac-IETD-CHO)(PartNo.90086):50μLof100μM(0.05mg/mL)inDMSO
      • pNAStandard(PartNo.90085):250μLof10mMinDMSO
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStorekitmaterialsat-20°Cuptotheirexpirationdate.

      SpecialPrecautions:

      ·Afterthawingreagents,useimmediatelyoraliquotandfreezeat-20oCforlongerstorage.Avoidrepeatedfreeze/thawcycles.

      ·TheCaspase-8substrateandpNAstandardareespeciallylightsensitive.Maintainthesereagentsinamberorcoveredcontainers.
      Applications
      ApplicationCaspase-8ColorimetricActivityAssayKitsprovideasimple&convenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceIETD.
      KeyApplications
      • ActivityAssay
      BIOLOGicalInformation
      SpeciesReactivity
      • Mammals
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymescomposedofaprodomain,alargeproteasesubunit,andasmallproteasesubunit.Activationofcaspasesrequiresproteolyticprocessingatconservedinternalasparticresiduestogenerateaheterodimericenzymeconsistingofthelargeandsmallsubunits.ThisproteinisinvolvedintheprogrammedcelldeathinducedbyFasandvariousapoptoticstimuli.TheN-terminalFADD-likedeatheffectordomainofthisproteinsuggeststhatitmayinteractwithFas-interactingproteinFADD.ThisproteinwasdetectedintheinsolublefractionoftheaffectedbrainregionfromHuntingtondiseasepatientsbutnotinthosefromnormalcontrols,whichimplicatedtheroleinneurodegenerativediseases.Manyalternativelysplicedtranscriptvariantsencodingdifferentisoformshavebeendescribed,althoughnotallvariantshavehadtheirfull-lengthsequencesdetermined.
      GeneSymbol
      • CASP8
      • MCH5
      • MGC78473
      • CASP-8
      • MACH
      • ALPS2B
      • procaspase-8
      • FLICE
      • CAP4
      • EC3.4.22.61[Contains:Caspase-8subunitp18
      • Caspase-8subunitp10].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q14790#MostupstreamproteaseoftheactivationcascadeofcaspasesresponsIBLefortheTNFRSF6/FASmediatedandTNFRSF1Ainducedcelldeath.BindingtotheadaptermoleculeFADDrecruitsittoeitherreceptor.Theresultingaggregatecalleddeath-inducingsignalingcomplex(DISC)performsCASP8proteolyticactivation.TheactivedimericenzymeisthenliberatedfromtheDISCandfreetoactivatedownstreamapoptoticproteases.ProteolyticfragmentsoftheN-terminalpropeptide(termedCAP3,CAP5andCAP6)arelikelyretainedintheDISC.CleavesandactivatesCASP3,CASP4,CASP6,CASP7,CASP9andCASP10.MayparticipateintheGZMBapoptoticpathways.CleavesADPRT.Hydrolyzesthesmall-moleculesubstrate,Ac-Asp-Glu-Val-Asp--AMC.LikelytargetforthecowpoxvirusCRMAdeathinhibitoryprotein.Isoforms5,6,7and8lackthecatalyticsiteandmayinterferewiththepro-apoptoticactivityofthecomplex.
      SIZE:479aminoacids;55391Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya18kDa(p18)anda10kDa(p10)subunit.InteractswithFADD,CFLARandPEA15.Isoform9interactsattheendoplasmicreticulumwithacomplexcontainingBCAP31,BAP29,BCL2and/orBCL2L1.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Isoforms1,5and7areexpressedinawidevarietyoftissues.Highestexpressioninperipheralbloodleukocytes,spleen,thymus,andliver.Barelydetectableinbrain,testis,andskeletalmuscle.DOMAIN:SwissProt:Q14790Isoform9containsaN-terminalextensionthatisrequiredforinteractionwiththeBCAP31complex.
      PTM:Generationofthesubunitsrequiresassociationwiththedeath-inducingsignalingcomplex(DISC),whereasadditionalprocessingislikelyduetotheautocatalyticactivityoftheactivatedprotease.GZMBandCASP10canbeinvolvedintheseprocessingevents.&PhosphorylateduponDNAdamage,probablybyATMorATR.
      DISEASE:SwissProt:Q14790#DefectsinCASP8arethecauseofcaspase-8deficiency(CASP8D)[MIM:607271].CASP8Disadisorderresemblingautoimmunelymphoproliferativesyndrome(ALPS).Itischaracterizedbylymphadenopathy,splenomegaly,anddefectiveCD95-inducedapoptosisofperipheralbloodlymphocytes(PBLs).ItleadstodefectsinactivationofT-lymphocytes,B-lymphocytes,andnaturalkillercellsleADIngtoimmunodeficiencycharacterizedbyrecurrentsinopulmonaryandherpessimplexvirusinfectionsandpoorresponsestoimmunization.
      SIMILARITY:BelongstothepeptidaseC14family.&Contains2DED(deatheffector)domains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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