Immunogen | Vimentinpurifiedfrombovinelens |
Clone | VIM3B4 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Mouse |
Specificity | Highlyspecificfortheintermediatefilamentproteinvimentinwhichispresentinallcellsofmesenchymalorigin.Thisantibodyispositivewithcellsofmesenchymalderivation,includingendothelialcellsandcertainsmoothmusclecellsofthevasculartract,fibroblasts,connectivetissue,alltypesofbloodcells,includingthymocytes,interstitialandtesticularSertolicells,ovarianfolliclecells.Itcanalsobeusedtodetectcoexpressionofvimentinincombinationwithotherintermediatefilamentproteins.VIM3B4hasturnedouttobethemostavidmonoclonalantibodytovimentin.Tumorsspecificallydetected:sarcoma(includingmyosarcoma),lymphoma,melanoma.ThebindingregionofmonoclonalantibodyVIM3B4hasbeencharacterizedbyBohnetal.(1992).Accordingtotheseauthors,theepitopehasbeenlocalizedonthealpha-helicalpartofvimentin(roddomaincoil2).Duetoanaasubstitutionatpositionofaa353inmurinevimentin(thatcouldexplainfortheweakcross-reactionoftheantibodywithmurinevimentin)theywereabletonarrowdownthebindingregionaroundposition353.Thesefindingswereconfirmedbytruncationmutagenesisexperimentsusinghumanvimentin(Rogersetal.,1995).Polypeptidereacting:MW57kDaintermediatefilamentprotein(vimentin)ofmesenchymalcells. |
Isotype | IgG2a |
SpeciesReactivity | - Amphibian
- Bovine
- Canine
- Chicken
- Human
- Monkey
|
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | AlongwiththeMICROFILaments(actins)andmicrotubules(tubulins),theintermediatefilamentsrepresentathirdclassofwell-characterizedcytoskeletalelements.Thesubunitsdisplayatissue-specificpatternofexpression.Desmin(MIM125660)isthesubunitspecificformuscleandvimentinthesubunitspecificformesenchymaltissue.[suppliedbyOMIM] |
GeneSymbol | - VIM
- FLJ36605
- Vimentin.
- vimentin
|
PurificationMethod | ProteinAPurfied |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P08670#Vimentinsareclass-IIIintermediatefilamentsfoundinvariousnon-epithelialcells,especiallymesenchymalcells. SIZE:466aminoacids;53652Da SUBUNIT:Homopolymer.ComponentofacomplexcomposedatleastofACTB,AP2M1,AP2A1,AP2A2,MEGF10andVIM.InteractswithHCVcoreprotein. TISSUESPECIFICITY:Highlyexpressedinfibroblasts,someexpressioninT-andB-lymphocytes,andlittleornoexpressioninBurkitt"slymphomacelllines.Expressedinmanyhormone-independentmammarycarcinomacelllines. PTM:Oneofthemostprominentphosphoproteinsinvariouscellsofmesenchymalorigin.Phosphorylationisenhancedduringcelldivision,atwhichtimevimentinfilamentsaresignificantlyreorganized. SIMILARITY:SwissProt:P08670##Belongstotheintermediatefilamentfamily. |
MolecularWeight | 57kDa. |