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Millipore/CC119 | Human Caspase 3, recombinant human active/CC119/25 units
  • Millipore/CC119 | Human Caspase 3, recombinant human active/CC119/25 units

Millipore/CC119 | Human Caspase 3, recombinant human active/CC119/25 units

價(jià)格: ￥2580.00 市場價(jià): 4300.00
貨號: CC119
品牌: Millipore
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    • Description
      CatalogueNumberCC119
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionHumanCaspase3,recombinanthumanactive
      OverviewSpecificity:Caspase-3(alsoknownasCPP32,Yamaandapopain)isamemberoftheinterleukin-1betaconvertingenzyme(ICE)familyofcysteineproteases.Caspase-3existsincellsasaninactive32kDaproenzyme.Duringapoptosisprocaspase-3isprocessedataspartateresiduesbyself-proteolysisand/orcleavagebyupstreamcaspases,suchascaspase-6(Mch2),caspase-8(FLICE)granzymeB.Theprocessedformofcaspase-3consistsoflarge(17kDa)andsmall(12kDa)subunitswhichassociatetoformtheactiveenzyme.Theactivecaspase-3hasbeenshowninvolvingintheproteolysisofseveralimportantmolecules,suchaspoly(ADP-ribose)polymerase(PARP),thesterolregulatoryelementbindingproteins(SREBPs),focaladhesionkinase(FAK),andothers.

      Thepartiallypurified*activerecombinanthumancaspase-3proteinwithfull-lengthHis-6tagwasexpressedinE.coli.TheexpressedCaspase-3spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Therecombinantcaspase-3preferentiallycleavescaspase-3substrates(e.g.,DEVD-AFCorDEVD-pNA).Incombinationwithcaspase-3activityassays,theactiverecombinantcaspase-3isveryusefulintheBIOLOGicalscreeningofcaspaseinhibitors.Therecombinantenzymecanalsobeusedasapositivecontrolincaspaseassaysorindeterminingthespecificityofcaspasesubstrates.

      *10-25%caspase-3proteinbygelanalysis,alsocontainsE.Coliproteins.Approximately,0.8-2μgtotalprotein/unitofactivity.GenBank#U13737.
      ProductInformation
      PresentationLyophilized.Reconstituteto1unit/μLwithPBScontaining15%glycerol.

      Partiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase3protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity.
      StorageandShippingInformation
      StorageConditionsMaintainunopenedandrehydratedproductat-70°Cinundiluted,freeze-thawprotected,tightlysealedaliquots.Avoidrepeatedfreeze/thawcycles.Productstablefor6monthsfromdateofpurchasedifhandledappropriately.
      Applications
      KeyApplications
      • ActivityAssay
      ApplicationNotesWesternblot:1-5μLofstockperlane.Proteinisfulllengthcaspase3withHis-Tag.Itisautoactivatedthusactivecaspasesubunitswillbeseen

      and17kDa+12kDa(largeandsmallsubunits)canbeseendependingupontheantibody.Smallsubunitisextremelydifficulttodetectinmostwesterns.Thesizeofinactiveenzimeis~32kDa.

      Activation:Notneeded.WhenexpressedinE.coli,caspase-3spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicoftheactiveenzyme.
      BiologicalInformation
      PurityPartiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase3protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity.
      SpecificActivityOneunitoftherecombinantcaspase-3istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateDEVD-pNA(pNA:pnitroanaline)perhourat37°Catsaturatedsubstrateconcentrations.
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.
      GeneSymbol
      • CASP3
      • apopain
      • CASP-3
      • procaspase3
      • CPP32B
      • SCA-1
      • CPP32
      • Apopain
      • CPP-32
      • Yama
      • EC3.4.22.56[Contains:Caspase-3subunitp17
      • Caspase-3subunitp12].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin.
      SIZE:277aminoacids;31608Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem.
      PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa.
      SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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