EGFR/CEN7 CISH Probe is designed for the qualitative detection of human EGFR gene amplifications as well as the detection of chromosome 7 alpha satellites in formalin-fixed, paraffinembedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:This Probe is composed of:1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 7p11.2* (chr7:55,082,262-55,278,647) harboring the EGFR gene region.2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 7p11.1-q11.1 specific for the alpha satellite centromeric region D7Z1 of chromosome 7. 3. Formamide based hybridization buffer.*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Interpretation of results:Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (EGFR gene region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 7).Normal situation: In interphases of normal cells or cells without an amplification involving the EGFR gene region, two distinct dot-shaped green and two distinct dot-shaped red signals appear.Aberrant situation: In cells with an amplification of the EGFR gene region, an increased number of green signals or green signal clusters will be observed.Overlapping signals may appear as brown signals. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
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Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
Normal nuclei each with two EGFR (green) and two centromere 7 (red) signals.
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