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Abnova/BCL6 Split CISH Probe/1kit/CS0003
  • Abnova/BCL6 Split CISH Probe/1kit/CS0003

Abnova/BCL6 Split CISH Probe/1kit/CS0003

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貨號(hào): CS0003
品牌: Abnova
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  • 詳情
  • 使用說明
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      • Specification
      • Gene Info
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • BCL6 Split CISH Probe is designed for the qualitative detection of translocations involving the human BCL6 gene at 3q27.3 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
      • Recommend Usage:
      • The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
      • Supplied Product:
      • Reagent Provided:This Probe is composed of:1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 3q27.3* (chr3:187,028,236-187,214,873) proximal to the BCL6 breakpoint region.2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 3q27.3-q28* (chr3:187,744,962-187,940,859) distal to the BCL6 breakpoint region .*according to Human Genome Assembly GRCh37/hg19
      • Storage Instruction:
      • Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
      • Note:
      • The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Interpretation of results:Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (proximal to the BCL6 breakpoint region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (distal to the BCL6 breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the BCL6 gene region, two red/green fusion signals appear.Aberrant situation: One BCL6 gene region affected by a translocation is indicated by one separate green signal and one separate red signal.Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
      • Probe Position:
      • Regulatory Status:
      • For research use only (RUO)
      • Interpretation of Result:
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • Chromogenic In Situ Hybridization (Cells)
      • Chromogenic <i>In Situ</i> Hybridization (Cells)
      • Normal interphase cells as indicated by two red/green fusion signals per nucleus.
      • Application Image
      • Chromogenic In Situ Hybridization (Cells)
      • Chromogenic <i>In Situ</i> Hybridization (Cells)
      • enlarge
      • Gene Information
      • Entrez GeneID:
      • 604
      • Gene Name:
      • BCL6
      • Gene Alias:
      • BCL5,BCL6A,LAZ3,ZBTB27,ZNF51
      • Gene Description:
      • B-cell CLL/lymphoma 6
      • Omim ID:
      • 109565
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq
      • Other Designations:
      • B-cell lymphoma 6 protein,B-cell lymphoma 6 protein transcript,cys-his2 zinc finger transcription factor,lymphoma-associated zinc finger gene on chromosome 3,zinc finger protein 51,zinc finger transcription factor BCL6S
      • Related Disease
      • Adenocarcinoma
      • Central Nervous System Neoplasms
      • Esophageal Neoplasms
      • Genetic Predisposition to Disease
      • Hematologic Diseases
      • HIV Infections
      • Hodgkin Disease
      • Kidney Failure, Chronic
      • Leukemia, chronic myeloid
      • Leukemia, Myelogenous, Chronic, BCR-ABL Positive
      • Lung Neoplasms
      • Lymphoma, AIDS-Related
      • Lymphoma, B-Cell
      • Lymphoma, Follicular
      • Lymphoma, Large B-Cell, Diffuse
      • Lymphoma, Large-Cell, Diffuse
      • Lymphoma, Non-Hodgkin
      • Lymphoproliferative Disorders
      • Neovascularization, Pathologic
          • ... see more
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