| Description: | The HotStart Taq DNA Polymerase is a chemically modified Taq DNA Polymerase, whose enzyme activities can only be activated after 3-5 minutes of incubation at 94oC. The HotStart Taq Polymerase uses amplification conditions for regular Taq DNA Polymerase, except no polymerase activity will be present before the onset of thermal cycling. This prevents nonspecific DNA amplification and primer dimer formation. The amplified products, up to 6 kb in length, contain single 3’-overhanging-A ends. This allows for TA cloning methods, if the amplified fragments need to be cloned. The enzyme is available in 200 and 500 unit sizes at a concentration of 5 U/μL. The enzyme is supplied with a 10× Reaction Buffer. |
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