產(chǎn)品概述:產(chǎn)品參數(shù) 外觀:可溶于DMSO或DMF的白色固體 Ex/Em(pH>6):504/529 nm(終產(chǎn)物) CAS號(hào):4091-99-0 分子式:C24H16Cl2O7 分子量:487.29儲(chǔ)存條件 -20℃ 干燥避光保存,有效期見外包裝。 產(chǎn)品介紹 H2DCFDA 是活性氧族的細(xì)胞滲透性指示劑,可在細(xì)胞內(nèi)的酯酶發(fā)生氧化反應(yīng)去除其乙酸基團(tuán)之前保持非熒光性。在氧化環(huán)境中,H2DCFDA 轉(zhuǎn)變成發(fā)出綠色熒光的 2,7-二氯二氫熒光素。注意事項(xiàng) 1. 在實(shí)驗(yàn)開始前,產(chǎn)品應(yīng)以高濃度貯存,并保持密封。 2. 實(shí)驗(yàn)中,在預(yù)熱的 PBS、HBSS 或 HEPES 等其他簡單的生理緩沖液中加入該產(chǎn)品,推薦工作濃度為 1-10 μM。
說明書:UE-D1002 使用本產(chǎn)品的文獻(xiàn):1.A multiple functional supramolecular system for synergetic treatments of hepatocellular carcinomaLijingSun, LiyuanChen, KeYang, Wei FengDai, YeYang, XiumingCui, BoYang, ChengxiaoWangInternational Journalof Pharmaceutics參考文獻(xiàn)1.Anticonvulsant Valproic Acid Inhibits Cardiomyocyte Differentiation of Embryonic Stem Cells by Increasing Intracellular Levels of Reactive Oxygen Species應(yīng)用方向:組織內(nèi)活性氧(ROS)含量2.Oxysterol-Induced Toxicity in R28 and ARPE-19 Cells應(yīng)用方向:評(píng)估活性氧中間體(ROI)的生產(chǎn)3.Reactive Oxygen Species-Producing Site in Radiation and Hydrogen Peroxide-Induced Apoptosis of Human Peripheral T Cells: Involvement of Lysosomal Membrane Destabilization應(yīng)用方向:溶酶體中ROS的檢測(cè)4.Mitochondrial depolarization following hydrogen sulfide exposure in erythrocytes from a sulfide-tolerant marine invertebrate應(yīng)用方向:自由基指示劑