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Abnova/CDK2(phospho T14) & CDK2 Protein Phosphorylation Antibody Pair/1kit/DP0075
  • Abnova/CDK2(phospho T14) & CDK2 Protein Phosphorylation Antibody Pair/1kit/DP0075

Abnova/CDK2(phospho T14) & CDK2 Protein Phosphorylation Antibody Pair/1kit/DP0075

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貨號(hào): DP0075
品牌: Abnova
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  • 詳情
  • 使用說明
  • 常見問題
      • Specification
      • Gene Info
      • Interactome
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • This protein phosphorylation antibody pair set comes with two antibodies, one against the CDK2 protein, and the other against the specific T14 phosphorylated site of CDK2 for use in in situ Proximity Ligation Assay. See Publication Reference below.DuoLink
      • Reactivity:
      • Human
      • Quality Control Testing:
      • Dual recognition immunofluorescence result.QC Testing of DP0075Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
      • Supplied Product:
      • Antibody pair set content: 1. Phospho-CDK2 T14 rabbit polyclonal antibody (20 ul) In PBS (0.09% (w/v) sodium azide) 2. CDK2 mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
      • Storage Instruction:
      • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
      • 2.
      • Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
      • 3.
      • Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
      • 4.
      • Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.
      • 5.
      • Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009
      • Applications
      • In situ Proximity Ligation Assay (Cell)
      • Application Image
      • In situ Proximity Ligation Assay (Cell)
      • Gene Information
      • Entrez GeneID:
      • 1017
      • Gene Name:
      • CDK2
      • Gene Alias:
      • p33(CDK2)
      • Gene Description:
      • cyclin-dependent kinase 2
      • Omim ID:
      • 116953
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
      • Other Designations:
      • cdc2-related protein kinase,cell devision kinase 2,p33 protein kinase
      • Interactome
      • Interactome
      • Gene Pathway
      • Cell cycle
      • p53 signaling pathway
      • Pathways in cancer
      • Prostate cancer
      • Small cell lung cancer
      • Related Disease
      • Azoospermia
      • Breast cancer
      • Breast Neoplasms
      • Chromosome Aberrations
      • Diabetes Mellitus, Type 1
      • Genetic Predisposition to Disease
      • Kidney Failure, Chronic
      • Lymphoma, Mantle-Cell
      • Neoplasm Invasiveness
      • Ovarian cancer
      • Ovarian Neoplasms
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