當前位置 ： NEB >>> NEB/NEBExpress? MBP Fusion and Purification System/1 set/E8201S
NEB/NEBExpress? MBP Fusion and Purification System/1 set/E8201S
  • NEB/NEBExpress? MBP Fusion and Purification System/1 set/E8201S

NEB/NEBExpress? MBP Fusion and Purification System/1 set/E8201S

價格: 試用 市場價: 0.00
貨號: E8201S
品牌: NEB
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品：無質(zhì)量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費服務(wù)熱線

4000-520-616

  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價
  • 品質(zhì)甄選 正品保證
  • 嚴控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • In the NEBExpress® MBP Fusion and Purification System, the pMAL-c6T vector provides a method for expressing and purifying a protein produced from a cloned gene or open reading frame. The cloned gene is inserted downstream from and in frame with the malE gene of E. coli, which encodes maltose-binding protein (MBP); this construct results in the expression of an MBP fusion protein (2,3). The pMAL-c6T vector expresses the N-terminal hexahistidine tagged malE gene (lacking its secretory signal sequence and engineered for tighter binding to amylose) followed by a multiple cloning site containing a TEV protease recognition sequence and stop codons in all three frames. The pMAL-c6T vector expresses the MBP fusion in the cytoplasm. The method uses the strong “tac” promoter and the malE translation initiation signals to yield high-level expression of the cloned sequences (4,5). The fusion protein is then purified by a one-step purification method using amylose resin and MBP’s affinity for maltose(6).Following amylose purification, the target protein can be cleaved from the MBP-tag using TEV Protease, without adding any vector-derived residues to the protein. Both the MBP-tag and TEV Protease are polyhistidine-tagged for easy removal from the reaction. Loading the digest onto NEBExpress Ni Resin (NEB #S1428) sequesters both the MBP-tag and TEV Protease, thereby isolating the target protein in the column flow through. The target protein yield can be up to 100 mg/L, with typical yields in the range of 10–40 mg/L.
      Figure 1: Schematic illustration of the NEBExpress MBP Fusion and Purification System
      Figure 2. Protein Expression using the NEBExpress MBP Fusion and Purification System
      SDS-polyacrylamide gel electrophoresis of fractions from the affinity purification of MBP6-TEV-Paramyosin ΔSal. Lane 1: Protein Standard. Lane 2: uninduced cells. Lane 3: induced cells. Lane 4: purified fusion protein eluted from amylose column with maltose. Lane 5: purified protein after TEV Protease cleavage. Lane 6: target protein isolated from NEBExpress Ni Resin flow through.
      This product is related to the following categories:
      Amylose Purification (MBP-tag),
      NEBExpress MBP Fusion and Purification System,
      Non-T7 Expression,
      Bacterial E. coli Protein Expression,
      Affinity Purification,
      Protein Purification,
      E. coli Expression Strains Products,
      Protein Expression
      This product can be used in the following applications:
      Non-T7 Expression,
      Target Protein Insolubility ,
      Expression of Difficult Proteins,
      Protein Expression in E. Coli,
      Protein Expression
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態(tài)度，由螞蟻淘公司專業(yè)人員負責品控、采購、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關(guān)，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時免費服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯了怎么辦？
    A：同個訂單購買多個商品可能會分為一個以上包裹發(fā)出，可能不會同時送達，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個月內(nèi)（以快遞公司顯示簽收時間為準），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內(nèi)申請退換貨；特殊商品以合同條款為準。

何為188

極簡而嚴謹，我們僅銷售188款生物醫(yī)學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號

獲取最新優(yōu)惠活動通知

  • 品質(zhì)甄選，正品保證

  • 自營電商，廠家直采

  • 極簡主義，188精品