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Abnova/mutaFISH™ AR-V7 ARwt RNA Probes/1kit/FP0004
  • Abnova/mutaFISH™ AR-V7 ARwt RNA Probes/1kit/FP0004

Abnova/mutaFISH™ AR-V7 ARwt RNA Probes/1kit/FP0004

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貨號(hào): FP0004
品牌: Abnova
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  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
      • Specification
      • Gene Info
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • mutaFISH™ AR-V7 ARwt RNA Probes is designed to detect AR wildtype and AR-V7 on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
      • Supplied Product:
      • Content:1. RT AR-V7 Primer2. RT ARwt Primer3. mutaFISH™ AR-V7 RNA Probe4. mutaFISH™ ARwt RNA Probe5. Detection Probe-Aqua 4316. Detection Probe-Texas Red X
      • Storage Instruction:
      • Store at -20°C.Aliquot to avoid repeated freezing and thawing.
      • Note:
      • We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
      • Protocol:
      • PDF DownloadProtocol Download
      • Technology:
      • mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
      • Comparison:
      • FISH Probes vs mutaFISH™ Probes
      • Fluorophore:
      • Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
      • Probe Position:
      • Regulatory Status:
      • For research use only (RUO)
      • Video:
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • mutation specific, Fluorescence In Situ Hybridization (Cells)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Cells)
      • Fig.1 mutaFISH™ staining was performed in situ in human CWR22Rv1 cells captured by CytoQuest™ CR. AR-V7 was detected via orange signal (Cy3), and ARwt was detected via red signal (Cy5).Fig.2 mutaFISH™ staining was performed in situ in human PBMC cells. Both AR-V7 and ARwt were not detected.
      • mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Frozen Tissue)
      • mutaFISH™ staining was performed in situ in human frozen tissue from breast cancer. ARwt was detected via red signal (Cy5), and AR-V7 was detected via orange signal (Cy3).
      • Application Image
      • mutation specific, Fluorescence In Situ Hybridization (Cells)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Cells)
      • enlarge
      • mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Frozen Tissue)
      • enlarge
      • Gene Information
      • Entrez GeneID:
      • 367
      • Gene Name:
      • AR
      • Gene Alias:
      • AIS,DHTR,HUMARA,KD,NR3C4,SBMA,SMAX1,TFM
      • Gene Description:
      • androgen receptor
      • Omim ID:
      • 176807, 300068, 300633, 313200, 313700
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq
      • Other Designations:
      • OTTHUMP00000023450,OTTHUMP00000061928,dihydrotestosterone receptor
      • Gene Pathway
      • Pathways in cancer
      • Prostate cancer
      • Related Disease
      • Abortion, Habitual
      • Acne Vulgaris
      • Adenocarcinoma
      • Adrenal Hyperplasia, Congenital
      • Alcoholism
      • Alopecia
      • Alzheimer Disease
      • Alzheimer disease
      • Androgen-Insensitivity Syndrome
      • Angina Pectoris, Variant
      • Antisocial Personality Disorder
      • Arteriosclerosis
      • Arthritis, Rheumatoid
      • Asperger Syndrome
      • Atherosclerosis
      • Attention Deficit Disorder with Hyperactivity
      • Autistic Disorder
      • Azoospermia
      • Biliary Tract Neoplasms
          • ... see more
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