Echelon’s Class III PI3K ELISA Kit is a competitive (96-well) ELISA assay kit that measures Class III Phosphoinositide 3-kinases activity directly through its reaction product, PtdIns(3)P (PI(3)P).
Product BackgroundThe Class III PI3K ELISA Kit is a competitive ELISA in which the signal is inversely proportional to the amount of PI(3)P produced. After the PI3-K reactions are complete, reaction products are added to the PI(3)P-coated microplate, for competitive binding to a PI(3)P detector protein. The amount of PI(3)P detector protein bound to the plate is determined through colorimetric detection.