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Pierce/c-Met (Soluble) Human ELISA Kit/96 tests/KHO2031
  • Pierce/c-Met (Soluble) Human ELISA Kit/96 tests/KHO2031

Pierce/c-Met (Soluble) Human ELISA Kit/96 tests/KHO2031

價格: ￥5388.00 市場價: 8980.00
貨號: KHO2031
品牌: Pierce
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    • Description

      TheHumanc-Met(Soluble)ELISAresearch-use-onlykitisdesignedtodetectandquantifythelevelofsolublehumanc-Metproteininserum,plasma,andcellculturesupernatantsusing96-wellplatesandamicroplatereader.Theassaywillrecognizebothnaturalandrecombinantsolublehumanc-Met.

      Performancecharacteristics
      •Sensitivity:<0.5>
      •Standardcurverange:0.78–50ng/mL
      •Sampletypes:serum,plasma,cellculturesupernatants
      •Speciescross-reactivity:human
      •Samplevolume:100μL(1:2to1:100)
      •Totalassayincubationtime:4hrs

      Principleofthemethod
      TheHumanc-Met(Soluble)kitisasolidphasesandwichenzyme-linkedimmunosorbentassay(ELISA).Ahighlypurifiedantibodyhasbeencoatedontothewellsofthemicrotiterstripsprovided.Duringthefirstincubation,standardsofknownsolublehumanc-Metcontent,controls,andunknownsamplesarePipettedintothewells.Afterwashing,biotinylatedsecondaryantibodyisadded.Afterwashing,streptavidin-peroxidase(enzyme)isadded.Thisbindstothebiotinylatedantibodytocompletethefour-membersandwich.Afterathirdincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionisadded,whichisacteduponbytheboundenzymetoproducecolor.Theintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofsolublehumanc-Metpresentintheoriginalspecimen.

      Backgroundinformation
      c-Met,amemberofthetyrosinekinasesuperfamily,isthereceptorforhepatocytegrowthfactor,alsoknownasscatterfactor(HGF/SF).Cellsexpressingc-Metincludeepithelialcells,endothelialcells,bloodcellsofvarioustypes,andglomerularmesenchymalcells.SourcesofHGF/SFincludemesenchymalcells,mesanglialcells,endothelialcells,macrophages,andtumorcells.

      HGF/SFbindingtoc-Metstimulatesreceptordimerizationandthephosphorylationofnumerousresidueswithinthereceptor’scytoplasmicdomain.Phosphorylationoftyrosines1234and1235ofc-Metisrequiredforactivationofthereceptor’styrosinekinaseactivity.c-Metphosphorylationalsogeneratesdockingsitesfornumeroussignalingmoleculesandstimulatesreceptorinternalizationviaclathrin-coatedvesicles.Signalingproteinsthatarephosphorylatedand/orlocalizedinresponsetoc-Metphosphorylationinclude:Grb2,Shc,Cbl,Crk,cortactin,paxillin,GAB1,PI-3K,FAK,Src,Ras,ERK1and2,JNK,PLC-γ,AKT,andSTAT3.HGF/SFstimulationofc-Metexpressingcellsenhancesproliferation,migration,morphogenesis,andproteasesynthesis,characteristicsthatareassociatedwithinvasivecellphenotype.

      Solublec-Metisatruncatedformofthec-Metmembranereceptor.Itcanbecleavedbyproteasesandreleasedfromthelipidbilayerinaprocessknownasectodomainshedding.Manytransmembraneproteinsarereleasedthroughthissheddingprocessanditisanormalpartofdevelopmentwhichwhendefectivecancauseanumberofpathologies.Thesolubleformofthec-Metreceptorissmallerthanthemembraneboundreceptor,containstheextracellularregionofthereceptor,andisabletobindtheHGFligand.

      Detectlow-expressingproteinsanduselesssample
      ELISAkitsdesignedtomeasureintracellularsignalingtargetsaretypically2–10timesmoresensitivethanwesternblotting.Theimprovedsensitivityenablesyoutodetectlow-expressingproteinsthatotherwisemaynotbedistinguishablefrombackground.Inaddition,theamountofsampleneededtoruntheassayislessthanwhatisneededforwesternblots.

      EachELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeproductinsertformoreinformationonvalidation.

      Relatedlinks
      LearnmoreaboutELISAkits
      Learnmoreaboutotherimmunoassays
      ForResearchUseOnly.Notforuseindiagnosticprocedures.

      Specifications

      ProteinFamily:Kinases&Inhibitors
      AssayIncubationTime:4hr
      AssayRange:0.78-50ng/mL
      AssaySensitivity:<0.5?ng/mL
      DetectionMethod:Colorimetric
      ForUseWith(Equipment):MicroplateReader
      FormerIdentifiers:Novex
      GeneAliases:AUTS9,DFNB97,HGFR,RCCP2,c-Met
      GeneID:4233
      GeneName:METproto-oncogene,receptortyrosinekinase
      GeneSymbol:MET
      ImmunoassayKitFormat:CoatedELISAKit
      LabelorDye:HRP(HorserADIshPeroxidase)
      ProductSize:96tests
      ProteinName:Hepatocytegrowthfactorreceptor
      ProteinSubtype:ReceptorTyrosineKinases
      SampleType(Specific):CellCultureSupernatants, Plasma, Serum
      SampleVolume:100µL
      ShippingCondition:WetIce
      Target(KitNamed):c-Met
      TargetSpecies(Named):Human
      TargetSpecies(Validated):Human
      TargetSub-Specificity:Soluble
      UniProtID:P08581

      Contents&storage

      Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.SeeproductmanualfordetailedcontentsandstorageconditionsformaximumstABIlity.
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