當(dāng)前位置 ： NEB >>> NEB/LyoPrime Luna? Probe One-Step RT-qPCR Mix with UDG/120 reactions/L4001S
NEB/LyoPrime Luna? Probe One-Step RT-qPCR Mix with UDG/120 reactions/L4001S
  • NEB/LyoPrime Luna? Probe One-Step RT-qPCR Mix with UDG/120 reactions/L4001S

NEB/LyoPrime Luna? Probe One-Step RT-qPCR Mix with UDG/120 reactions/L4001S

價(jià)格: 試用 市場價(jià): 0.00
貨號(hào): L4001S
品牌: NEB
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品：無質(zhì)量問題不接受退換貨，下單前請仔細(xì)核對信息。
下單后請及時(shí)聯(lián)系客服核對商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費(fèi)服務(wù)熱線

4000-520-616

  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • The LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use. By simply adding water, the mix can be rehydrated and is ready for use. It is designed for real-time detection of target RNA sequences using hydrolysis probes. Performance in multiplexing applications has been optimized, with sensitive, linear detection of up to 5 targets across a range of inputs. The stable cake can be resuspended to make a 2X or 4X mix to accommodate a variety of sample input volumes.
      Figure 1: LyoPrime Luna offers a robust, versatile RT-qPCR option in a shelf-stable lyophilized format
      The LyoPrime Luna™ RT-qPCR Mix (NEB #L4001) offers the same versatile features as the Luna RT-qPCR 4X Mix (NEB #M3019) in a lyophilized format that can be shipped and stored at room temperature.
      The mix contains the necessary components for one-step RT-qPCR, including Luna WarmStart RT, Hot Start Taq DNA Polymerase, dNTPs, and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start Taq DNA Polymerase with a WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps prevent undesirable non-specific priming and extension prior to thermocycling, enabling room temperature reaction set-up after rehydration. The engineered Luna WarmStart RT also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. Additionally, the mix is formulated with a unique passive reference dye that is compatible across a variety of instrument platforms, including those that require a high or low ROX reference signal.The sensitivity of RT-qPCR makes it important to minimize DNA contamination wherever possible. The inclusion of dUTP and thermolabile UDG prevents carryover contamination, where a product of a previous amplification can serve as the substrate of a subsequent reaction. Unlike E.coli UDG, the thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on qPCR performance.A non-fluorescent visible blue tracking dye is included to avoid pipetting errors. The tracking dye does not overlap spectrally with fluorophores commonly used in qPCR and does not interfere with real-time detection.To learn about the lyophilization process, or to inquire about customizing this product to meet your assay needs, please visit www.neb.com/lyoprime.
      Figure 2:Lyophilized and liquid Luna RT-qPCR mixes demonstrate equivalent strong performance
      A. RT-qPCR targeting human β-actin was performed using either the LyoPrime™ Luna Probe One-Step RT-qPCR Mix with UDG (NEB #L4001) or Luna® Probe One-Step 4X Mix with UDG (NEB #M3019) over an 8-log range of input template concentrations (1 μg – 0.1 pg Jurkat total RNA) with 4 replicates at each concentration, run on an ABI® QuantStudio™ 6 Flex real-time instrument. Reactions (20 μl) included primers at 400 nM each and and probe at 200 nM, and followed the product recommended cycling conditions. B. Standard Curve results were substantially equivalent for the lyophilized (gold) and liquid-format (blue) mixes, with strong linearity and reproducibility observed, even at the lowest concentrations tested.
      Figure 3:LyoPrime Luna enables robust multiplexing
      Multiplex RT-qPCR was performed using the LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG over a 6-log range of Jurkat total RNA (1 μg to 10 pg) on a Bio-Rad® CFX96 real-time instrument. Amplification standard curves and efficiencies for each of the 5 human targets are shown. Reactions (20 μl) included primers and probes at 200 nM each, and followed the product recommended cycling conditions. All five targets were detected linearly in the multiplex reactions with strong efficiency and R2 values.
      Figure 4:LyoPrime Luna matches the performance of the liquid Luna reagent and outperforms other commercial lyophilized mixes
      Commercially-available lyophilized RT-qPCR reagents were tested on 8 human RNA targets varying in abundance, length, and %GC. Data was collected by 2 users and according to manufacturers’ recommendations. Results were evaluated for efficiency, low input detection and lack of non-template amplification (where ΔCq = average Cq of non-template control – average Cq of lowest input). In addition, consistency, reproducibility and overall curve quality were assessed (Quality Score). The LyoPrime Luna Probe RT-qPCR Mix with UDG (NEB #L4001) outperformed other lyophilized products, with strong results comparable to those of the liquid format Luna Probe RT-qPCR Mix with UDG (NEB #M3019).Learn more about our comprehensive qPCR/RT-qPCR testing and “dots in boxes” data visualization here.
      Figure 5:Lyophilized and liquid Luna reagents demonstrate sensitive detection of SARS-CoV-2 viral RNA
      Multiplex RT-qPCR was performed using primers and probes from the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB #E3019) targeting 2019-nCoV_N1 (N1, HEX™), 2019-nCoV_N2 (N2, FAM™) and human RNase P (RP, Cy5), run on a Bio-Rad® CFX384 real-time instrument. Reactions (5 μl) included primers at 400 nM each and and probes at 200 nM each, and followed the product recommended cycling conditions. Performance was evaluated using Synthetic Twist SARS-CoV-2 RNA Control 2 diluted in 5 ng/μl human Jurkat total RNA. The Luna and LyoPrime Luna RT-qPCR Mixes both exhibited linear quantitation (standard curves of 100,000 to 10 copies SARS-CoV-2 RNA, left) and sensitive detection (100% detection of N1 and N2 with 40 replicates at 5 and 10 copies SARS-CoV-2 RNA; no false positive detection with 28 negative control replicates). Similar results were obtained for 20 μl reactions in 96-well format.
      Figure 6:LyoPrime Luna reagents enable strong detection and quantitation of various infectious disease targets
      Duplex RT-qPCR was performed using primers and probes targeting the indicated viral RNA gene (FAM) and human RNase P (Cy5) on a Bio-Rad CFX96 instrument. Performance was evaluated for triplicate standard curves of 100,000 to 10 copies ATCC® synthetic viral RNA diluted in 5 ng/μl human (Jurkat) total RNA (Chikungunya Virus: VR-3246SD; Dengue-2 Virus: VR-3229SD; Zika Virus: VR-3252SD). For each target the corresponding ATCC reference primer and probe sequences were used. Reactions (20 μl) included primers at 400 nM each and probes at 200 nM and followed the product recommended cycling conditions. The LyoPrime Luna RT-qPCR Mix exhibited linear quantitation and sensitive detection for each target.
      This product is related to the following categories:
      Luna? qPCR & RT-qPCR Products
      This product can be used in the following applications:
      qPCR & RT-qPCR,
      DNA Amplification, PCR & qPCR
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團(tuán)隊(duì)負(fù)責(zé)國際物流和清關(guān)，中間沒有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請?jiān)谑盏截?4小時(shí)內(nèi)申請退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢梗劢拐邕x每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知