當(dāng)前位置： NEB >>> NEB/SplintR? Ligase/6,250 units/M0375L

NEB/SplintR? Ligase/6,250 units/M0375L

價格: 試用市場價: 0.00

打印產(chǎn)品信息

貨號: M0375L

品牌: NEB

規(guī)格

默認

數(shù)量

庫存(0)

特別提示

代購產(chǎn)品：無質(zhì)量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格，訂單生效后再付款。

立即申請

收藏商品

資深產(chǎn)品顧問

咨詢顧問

全國免費服務(wù)熱線

4000-520-616

自營商城一站式服務(wù)
廠家直采剔除溢價
品質(zhì)甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

- SplintR Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand. This previously unreported activity may enable novel approaches for characterization of miRNAs and mRNAs, including SNPs. SplintR is ideally suited for many target enrichment workflows with applications in next-generation sequencing and molecular diagnostics. The robust activity of the enzyme and its affinity for RNA-splinted DNA substrates (apparent Km = 1 nM) enable sub-nanomolar detection of unique RNA species within a complex mixture, making SplintR ligase a superior choice for demanding RNA detection technologies.The enzyme is active over a broad range of ATP concentrations (10 µM – 1 mM) and pH (6.5–9). Optimal activity is observed using Mg²⁺ > 5 mM, and a pH between 7.5 and 8.0. The activity is enhanced at higher temperatures (up to 37°C), and by supplementation with 5 mM Mn²⁺. The reaction is inhibited by salt concentrations in excess of 100 mM.The enzyme tolerates all base pair combinations at the ligation junction, but is partially inhibited by dC/G and dG/C base pairs at the donor (phosphorylated) side ligation junction, particularly when the +2 base was also a C/G base pair.
  Ligation of DNA splinted by RNA
  (A) Outline of the ligation assay: a 5´-phosphorylated, 3´-FAM labeled DNA “donor” oligonucleotide and an unmodified DNA “acceptor” oligonucleotide areannealed to a complementary RNA splint. This substrate is reacted with a ligase to form a mixture of unreacted starting material (I), adenylylated DNA (II), and ligated product (III). Theseproducts are denatured, separated by capillary electrophoresis and detected by fluorescence. (B) Ligation of the RNA-splinted substrate in SplintR Ligase Reaction Buffer for 15 minutes at25°C with (a) no enzyme, (b) 1 μM T4 DNA Ligase and (c) 100 nM SplintR Ligase. Indicated peaks correspond to starting pDNA (I), AppDNA (II) and ligated product (III) as determined byco-elution with synthetically prepared standards. (C) The fraction of ligated product catalyzed by either SplintR Ligase or T4 DNA Ligase was analyzed by performing sets of ligations withboth ligases at concentrations between 10 pM and 10 μM for 15 minutes at 25°C. SplintR Ligase is clearly much more efficient at ligation of RNA splinted DNA than T4 DNA Ligase.
  Product Source
  An E. coli strain that carries a recombinant gene encoding PBCV-1 DNA Ligase.
  This product is related to the following categories:
  RNA Isolation & Enrichment Products,
  DNA Ligases Products
  This product can be used in the following applications:
  Small RNA Detection and Isolation,
  RNA Analysis
售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態(tài)度，由螞蟻淘公司專業(yè)人員負責(zé)品控、采購、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。

Q：生物188產(chǎn)品正品保障嗎？
A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責(zé)國際物流和清關(guān)，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進行修改；客服電話：4000-520-616

Q：可以開發(fā)票嗎？
A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發(fā)貨？
A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達！

Q：如何聯(lián)系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

Q：收到的商品少了/發(fā)錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發(fā)出，可能不會同時送達，建議查看訂單詳情是否是部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個月內(nèi)（以快遞公司顯示簽收時間為準），包裝規(guī)格、數(shù)量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內(nèi)申請退換貨；特殊商品以合同條款為準。