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NEB/LongAmp? Hot Start Taq 2X Master Mix/100 reactions (50 μl)/M0533S
  • NEB/LongAmp? Hot Start Taq 2X Master Mix/100 reactions (50 μl)/M0533S

NEB/LongAmp? Hot Start Taq 2X Master Mix/100 reactions (50 μl)/M0533S

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貨號: M0533S
品牌: NEB
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    • LongAmp Hot Start Taq 2X Master Mix contains a unique blend of aptamer-based Hot Start Taq and Deep Vent® DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. The LongAmp® Hot Start Taq 2X Master Mix does not require a separate high temperature incubation step to activate the enzyme. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq DNA Polymerase (1). LongAmp® Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.

      Amplification of a 8 kb amplicon from varying amounts of Jurkat genomic DNA using LongAmp Hot Start Taq 2X Master Mix. Starting template amounts are indicated below the gel. Marker M is the NEB 2-Log DNA Ladder (NEB #N3200 ).

      Product Source

      An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.Unit Assay Conditions:1X ThermoPol™ Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200µg/ml activated Calf Thymus DNA.
      This product is related to the following categories:
      LongAmp? DNA Polymerases Products,
      Master Mixes Products
      This product can be used in the following applications:
      Hot Start PCR,
      Long Range PCR,
      Multiplex PCR,
      Specialty PCR,
      Routine PCR,
      PCR
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