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NEB/NEBNext? FFPE DNA Repair Mix/24 reactions/M6630S
  • NEB/NEBNext? FFPE DNA Repair Mix/24 reactions/M6630S

NEB/NEBNext? FFPE DNA Repair Mix/24 reactions/M6630S

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貨號: M6630S
品牌: NEB
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    • The NEBNext FFPE DNA Repair Mix contains enzymes and buffers that are optimized to repair FFPE DNA in next generation sequencing workflow. The mix can be used upstream of any next generation sequencing library construction method, for any sequencing platform.Table 1: Types of FFPE DNA damage and their ability to be repaired by the NEBNext FFPE DNA Repair Mix
      FFPE DAMAGE TYPEREPAIRED BY THE FFPE DNA ENZYME REPAIR MIX?
      Deamination of cytosine to uracilYes
      Nicks and gapsYes
      Oxidized basesYes
      Blocked 3´ endsYes
      DNA fragmentationNo
      DNA-protein crosslinksNo
      Figure 1:FFPE DNA repair increases NGS library yields
      An example of Agilent Bioanalyzer® traces of libraries prepared from stomach tumor FFPE DNA that was treated with the FFPE DNA Repair Mix, or was untreated, before library construction.Yield improvements of 10% to 458% have been observed.
      Figure 2. FFPE DNA repair increases NGS library yields
      FFPE DNA samples (25 ng or 50 ng) from a variety of tissue types were treated with the NEBNext FFPE DNA Repair Mix, or were untreated, before library construction. Library yields were measured using the Agilent Bioanalyzer, and ratios of library yields with and without treatment with the NEBNext FFPE DNA Repair Mix were calculated. Yield improvements with these samples ranged from 101% to 458%.
      Figure 3. FFPE DNA Repair improves sequence quality from FFPE samples and does not introduce new mutations
      Genomic DNA was extracted using standard DNA purification. Each DNA sample was treated with the NEBNext FFPE DNA Repair Mix, or was untreated, before library construction and Illumina® sequencing.Panel A: The effect of DNA repair on the percentage of mappable reads. Reads were mapped to the human genome (hg19) using BWA (1).Panel B: The effect of DNA repair on the percentage of reads that were properly paired from a paired-end sequencing protocol.

      Lot Control

      The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
      This product is related to the following categories:
      FFPE DNA,
      Next Generation Sequencing Library Preparation
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