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Abnova/AKT (phospho S473) monoclonal antibody, clone RM251/1kit/MAB14912
  • Abnova/AKT (phospho S473) monoclonal antibody, clone RM251/1kit/MAB14912

Abnova/AKT (phospho S473) monoclonal antibody, clone RM251/1kit/MAB14912

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貨號(hào): MAB14912
品牌: Abnova
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  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
      • Specification
      • Gene Info
      • Interactome
      • Related Products
      • List All
      • Specification
      • Product Description:
      • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human AKT.
      • Immunogen:
      • A synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT1.
      • Host:
      • Rabbit
      • Reactivity:
      • Human
      • Form:
      • Liquid
      • Purification:
      • Protein A affinity purification
      • Isotype:
      • IgG
      • Recommend Usage:
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)Western Blot (1:1000-1:2000)The optimal working dilution should be determined by the end user.
      • Storage Buffer:
      • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
      • Storage Instruction:
      • Store at -20°C.Aliquot to avoid repeated freezing and thawing.
      • Note:
      • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate) analysis of 293 cell with AKT (phospho S473) monoclonal antibody, clone RM251 (Cat # MAB14912). 293 cells grownin medium with serum, serum starved, or insulin treated.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with AKT (phospho S473) monoclonal antibody, clone RM251 (Cat # MAB14912).
      • Application Image
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • enlarge
      • AKT1
      • AKT2
      • AKT3
      • Gene Information
      • Entrez GeneID:
      • 207
      • Gene Name:
      • AKT1
      • Gene Alias:
      • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
      • Gene Description:
      • v-akt murine thymoma viral oncogene homolog 1
      • Omim ID:
      • 164730, 181500
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
      • Other Designations:
      • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
      • Gene Information
      • Entrez GeneID:
      • 208
      • Gene Name:
      • AKT2
      • Gene Alias:
      • PKBB,PKBBETA,PRKBB,RAC-BETA
      • Gene Description:
      • v-akt murine thymoma viral oncogene homolog 2
      • Omim ID:
      • 125853, 164731
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
      • Other Designations:
      • Murine thymoma viral (v-akt) homolog-2,rac protein kinase beta
      • Gene Information
      • Entrez GeneID:
      • 10000
      • Gene Name:
      • AKT3
      • Gene Alias:
      • DKFZp434N0250,PKB-GAMMA,PKBG,PRKBG,RAC-PK-gamma,RAC-gamma,STK-2
      • Gene Description:
      • v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
      • Omim ID:
      • 611223
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The protein encoded by this gene is a member of the AKT, also called PKB,serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq
      • Other Designations:
      • OTTHUMP00000037911,OTTHUMP00000037912,RAC-gamma serine/threonine protein kinase,protein kinase B gamma,serine threonine protein kinase, Akt-3,v-akt murine thymoma viral oncogene homolog 3
      • Interactome 1
      • Interactome
      • Interactome 2
      • Interactome
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