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Millipore/MAB347 | Anti-Growth Associated Protein 43 Antibody, clone 9-1E12/MAB347/50 µg
  • Millipore/MAB347 | Anti-Growth Associated Protein 43 Antibody, clone 9-1E12/MAB347/50 µg

Millipore/MAB347 | Anti-Growth Associated Protein 43 Antibody, clone 9-1E12/MAB347/50 µg

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貨號(hào): MAB347
品牌: Millipore
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    • Description
      CatalogueNumberMAB347
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-GrowthAssociatedProtein43Antibody,clone9-1E12
      AlternateNames
      • Neuromodulin
      • B-50Protein
      • GrowthAssociatedProtein43
      • neuromodulin
      BackgroundInformationGAP-43,acommonMarkerofdifferentiatingneurons,isexpressedatelevatedlevelsbydevelopingorregeneratingneuronsduringaxongrowth.WhileGAP-43isanintegralmembraneproteinassociatedwiththecytoplasmicsurfaceofaxonalgrowthcones(andsynapses),itisabsentfromdendriticgrowthcones.Inadultbrain,GAP-43isfoundinhighconcentrationinpresynapticareaswherememoryformationisthoughttooccur,suchasfrontalcortex,limbicsystemandhippocampus.PhosphorylationofGAP-43byPKC(atSer41onhuman,mouse,rat,andbovineGAP-43orSer42onchickenandXenopusGAP-43)isspecificallycorrelatedwithcertainformsofsynapticplasticity.
      ProductInformation
      FormatPurified
      Control
      • RatDRGtissuethathasbeensubjectedtoaspinalnerveligationandculturedneurons.
      PresentationProteinAPurifiedmouseimmunoglobulinIgG1in20mMsodiumphosphate,250mMNaCl,pH.7.6,with0.1%sodiumazideasapreservative.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cinundilutedforupto6monthsafterdateofreceipt.
      Applications
      ApplicationAnti-GrowthAssociatedProtein43Antibody,clone9-1E12detectslevelofGrowthAssociatedProtein43&hasbeenpublished&validatedforuseinIH,IP&WB.
      KeyApplications
      • Immunohistochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:
      0.1-0.5μg/mLofapreviousloton4%paraformaldehydeperfusedandfixedratcerebellumandcerebrumtissue.Theadditionof0.01-0.1%TritonX-100toincubationbufferwillincreasecellularpermeABIlity.

      Immunoprecipitation:
      Apreviouslotofthisantibodywasusedinimmunoprecipitation.
      5-10μg/mLinmembranepreparationsfrom16-18dayembryoniccorticalneuronalcultures.

      WesternBlotAnalysis:
      MAB347willdetectasingle43-48kDbandinwesternblotsofmembranefractionsofgrowingneurons.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenGAP-43purifiedfromratbrain
      Clone9-1E12
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityGAP-43(alsoknownasgrowthassociatedprotein-43,B-50,F1andpp46),regardlessoftheprotein’sphosphorylationstate.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Rat
      • Feline
      • Hamster
      SpeciesReactivityNoteRat,felineandhamster.
      Expectedtoreactwithhumanbasedonsequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgenehasbeentermeda"growth"or"plasticity"proteinbecauseitisexpressedathighlevelsinneuronalgrowthconesduringdevelopmentandaxonalregeneration.Thisencodedproteinisconsideredacrucialcomponentofaneffectiveregenerativeresponseinthenervoussystem.
      GeneSymbol
      • GAP43
      • PP46
      • B-50
      • GAP-43/B50
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P17677#Thisproteinisassociatedwithnervegrowth.Itisamajorcomponentofthemotilegrowthconesthatformthetipsofelongatingaxons.
      SIZE:238aminoacids;24803Da
      SUBUNIT:BindscalmodulinwithagreateraffinityintheabsenceofCa(2+)thaninitspresence(Bysimilarity).
      SUBCELLULARLOCATION:Cellmembrane;Peripheralmembraneprotein;Cytoplasmicside.Cellprojection,growthconemembrane;Peripheralmembraneprotein;Cytoplasmicside.Celljunction,synapse.Note=Cytoplasmicsurfaceofgrowthconeandsynapticplasmamembranes.
      PTM:PhosphorylationofthisproteinbyaproteinkinaseCisspecificallycorrelatedwithcertainformsofsynapticplasticity.
      SIMILARITY:SwissProt:P17677##Belongstotheneuromodulinfamily.&Contains1IQdomain.
      MolecularWeight43-48kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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