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Millipore/MAB3570 | Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone N1/5/MAB3570/100 µg
  • Millipore/MAB3570 | Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone N1/5/MAB3570/100 µg

Millipore/MAB3570 | Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone N1/5/MAB3570/100 µg

價格: ¥3576.00 市場價: 5960.00

貨號: MAB3570
品牌: Millipore
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    • Description
      CatalogueNumberMAB3570
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-MyosinAntibody,smoothmuscleheavychain,SM1&SM2,cloneN1/5
      ProductInformation
      FormatPurified
      Control
      • POSITIVECONTROL:

        Smoothmuscle(e.g.aterialtunicamedia).Negativecontrol:anynonmuscletissue(e.g.arterialtunicaadentitial).
      PresentationLiquidin0.02MPhosphatebufferwith0.25MNaCland0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8*Cinundilutedaliquotsupto6monthsafterdateofreceipt
      Applications
      ApplicationDetectMyosinusingthisAnti-MyosinAntibody,smoothmuscleheavychain,SM1&SM2,cloneN1/5validatedforuseinIP,WB,IC,IH(P).
      KeyApplications
      • Immunoprecipitation
      • WesternBlotting
      • Immunocytochemistry
      • Immunohistochemistry(Paraffin)
      ApplicationNotesWesternblot(seeapplicationnotesbelow).SuggestedblockingbufferisTBS-Tweenwith2%BSA.SuggesteddilutionbufferisTBS-Tweenwith0.05%sodiumazide.Preferredgelpercentageis5%(seeapplicationnotes).

      Immunohistochemistryonfrozenandparaffinembeddedtissuesections.Suggestedfixationforfrozentissuesectionsisacetonefixfor6minutesatroomtemperature.Forformalinfixedparaffinembeddedtissuesections:microwavein0.01Mcitratebuffer(pH6.0)for8-10minutes(notethatallmicrowavesdifferandadjustmentsmayneedtobemade)followwithenzymedigestion(0.01%pronasefor10mintues).Suggestedblockingagentisfetalbovineserum.Theantibodyhasalsobeenusedsuccessfullyonmethyl-Carnoyfixedtissue.

      Immunocytochemistry

      Immunoprecipitation.Suggestedextractionbufferis20mMTris-HCl,pH7.4,150mMNaCl,1%TritonX-100,0.1%SDS,0.5%deoxycholicacid-NaCland0.5mMPMSF.Finalreactionvolumeis1mLandsuggestedcaptureagentisagaroseconjugatedanti-mouseIgG.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      APPLICATIONNOTESFORMAB3570

      WESTERNBLOT

      Toachievegoodresolutionofmyosinheavychainisoformswithdistinctmolecularweight(200-2004kDa),thefollowingprocedureshouldbefollowed:1).Pyrophoshateextractionbufferforsamplepreparation(seebelow);runSDS-PAGEin5%gel.Important:forbetterresolutionoftheMHCbands,useelectrophoreticbufferwithpH8.2(i.e.0.1lessthanstandard),andprepareresolvinggel(5%)withpH9.0(not8.8asusual).Alsohelpthoroughdegasingoftheresolvinggelmixture(H2O,acrylamide,EDTA,pH9.0,before(!)addingSDS,TEMEDandAPS).RunSDS-PAGElongerthanafterthedyefrontrunsoff(use200kDaMWMarkersandletit"s200kDabandrunatleasttothemiddleof8X8gel(usingabigsizegel(notthemini-gel!)willenhancethequalityofMHCbandresolution).

      Pyrophosphateextractionbuffer:(40mMNa4P2O7x10H2O,1mMMgCl2,1mMEGTA(addKOHtodissolveEGTA),PMSF,pH9.5).Toextractacto-myosinfromtissues/cells,shakemincedtissueorcellsincoldextractionbuffer1hronicebath(0oC),centrifuge@10,000gfor10minat+2-8oC,takesupernatantandmixit1:1withstandardLaemmlisamplebuffer,boil,runSDS-PAGEin5%gel(seeabove).
      BIOLOGicalInformation
      ImmunogenCrudesmoothmuscleextractfromnormalhumanadultuterus.
      Epitopesmoothmuscleheavychain,SM1&SM2
      CloneN1/5(alsoreferredtoasSM-M5)
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificitySmoothmusclemyosin,heavychain.ByWesternblottheantibodyrecognizesaproteinof200-204kDa.
      IsotypeIgG1
      SpeciesReactivity
      • Bovine
      • Human
      • Pig
      • Rabbit
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • CCDC128
      • MGC111781
      • FLJ16566
      UniProtNumber
      UniProtSummarySIZE:780aminoacids;88314Da
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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