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Millipore/MAB377 | Anti-NeuN Antibody, clone A60/MAB377/500 µg
  • Millipore/MAB377 | Anti-NeuN Antibody, clone A60/MAB377/500 µg

Millipore/MAB377 | Anti-NeuN Antibody, clone A60/MAB377/500 µg

價(jià)格: ￥4440.00 市場(chǎng)價(jià): 7400.00
貨號(hào): MAB377
品牌: Millipore
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    • Description
      CatalogueNumberMAB377
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-NeuNAntibody,cloneA60
      AlternateNames
      • Neuron-SpecificNuclearProtein
      • Neuna60
      • A60
      BackgroundInformationNeuNantibody(NEUronalNuclei;cloneA60)specificallyrecognizestheDNA-binding,neuron-specificproteinNeuN,whichispresentinmostCNSandPNSneuronalcelltypesofallvertebratestested.NeuNproteindistributionsareapparentlyrestrictedtoneuronalnuclei,perikaryaandsomeproximalneuronalprocessesinbothfetalandadultbrainalthough,someneuronsfailtoberecognizedbyNeuNatallages:INLretinalcells,Cajal-Retziuscells,Purkinjecells,inferiorolivaryanddentatenucleusneurons,andsympatheticganglioncellsareexamples(Mullenetal.,1992;Wolfetal.,1996).ImmunohistochemicallydetectableNeuNproteinfirstappearsatdevelopmentaltimepointsthatcorrespondwiththewithdrawaloftheneuronfromthecellcycleand/orwiththeinitiationofterminaldifferentiationoftheneuron(Mullenetal.,1992).ImmunoreactivityappearsaroundE9.5inthemouseneuraltubeandisextensivethroughoutthedevelopingnervoussystembyE12.5.Strongnuclearstainingsuggestsanuclearregulatoryproteinfunction;however,noevidencecurrentlyexistsastowhethertheNeuNproteinantigenhasafunctioninthedistalcytoplasmorwhetheritismerelysynthesizedtherebeforebeingtransportedbackintothenucleus.Nodifferencebetweenproteinisolatedfrompurifiednucleiandwholebrainextractonimmunoblotshasbeenfound(Mullenetal.,1992).
      ProductInformation
      FormatPurified
      Control
      • Positivecontrol-BrainTissue.Negativecontrol-AnynonneuronaltissueegFibroblasts
      PresentationPurifiedmouseimmunoglobulinIgG1liquidinbuffercontaining0.02Mphosphatebuffer,0.25MNaCl,pH7.6with0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor6monthsat2-8oCfromdateofreceipt.
      Applications
      ApplicationAnti-NeuNAntibody,cloneA60detectslevelofNeuNandhasbeenpublishedandvalidatedforuseinFC,IC,IF,IH,IH(P),IPandWB.
      KeyApplications
      • FlowCytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesWesternBlotAnalysis:
      Apreviouslotofthisantibodyrecognized2-3bandsinthe46-48kDarangeandpossIBLyanotherbandatapproximately66kDa.

      Immunocytochemistry:
      1:10-1:100dilutionfromapreviouslotwasused.Neuronsincultureshouldbepermeablizedwith0.1%tritonX-100.Allprimaryantibodydilutionsshouldbeperformedwithsimplesolutionscontainingonlybufferandprimaryantibodywithoutexcessproteinblocksordetergents.

      Immunohistochemistry:
      1:100-1:1,000.Theantibodyworksbestonpolyesterwaxembeddedtissuebutalsoworksonparaffinembeddedtissueatalowerworkingdilution.Theantibodyworkswellwithformaldehyde-basedfixatives.Citricacidandmicrowavepretreatmenthasbeenusedsuccessfully(Sarnat,1998).

      Immunohistochemistry(paraffin)Analysis:ApreviouslotwasusedforIH(P).

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenPurifiedcellnucleifrommousebrain
      CloneA60
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityMillipore"sexclusivemonoclonalantibodytovertebrateneuron-specificnuclearproteincalledNeuN(orNeuronalNuclei)reactswithmostneuronalcelltypesthroughoutthenervoussystemofmiceincludingcerebellum,cerebralcortex,hippocampus,thalamus,spinalcordandneuronsintheperipheralnervoussystemincludingdorsalrootganglia,sympatheticchaingangliaandentericganglia.Developmentally,immunoreactivityisfirstobservedshortlyafterneuronshavebecomepostmitotic,nostaininghasbeenobservedinproliferativezones.Theimmunohistochemicalstainingisprimarilylocalizedinthenucleusoftheneuronswithlighterstaininginthecytoplasm.ThefewcelltypesnotreactivewithMAB377includePurkinje,mitralandphotoreceptorcells.TheantibodyisanexcellentMarkerforneuronsinprimaryculturesandinretinoicacid-stimulatedP19cells.Itisalsousefulforidentifyingneuronsintransplants.
      IsotypeIgG1
      SpeciesReactivity
      • Avian
      • Chicken
      • Ferret
      • Human
      • Mouse
      • Pig
      • Rat
      • Salamander
      AntibodyTypeMonoclonalAntibody
      GeneSymbol
      • NeuN
      • A58
      PurificationMethodProteinAPurfied
      MolecularWeight46/48kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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