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Abnova/5-mC monoclonal antibody, clone b/1kit/MAB4952
  • Abnova/5-mC monoclonal antibody, clone b/1kit/MAB4952

Abnova/5-mC monoclonal antibody, clone b/1kit/MAB4952

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貨號: MAB4952
品牌: Abnova
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      • Specification
      • Product Description:
      • Mouse monoclonal antibody raised against 5-methyl Cytosine.
      • Immunogen:
      • Ovalbumine conjugated with 5-methyl Cytosine.
      • Host:
      • Mouse
      • Reactivity:
      • Human, Mouse
      • Form:
      • Liquid
      • Isotype:
      • IgG1
      • Recommend Usage:
      • ELISA (0.1-0.3 ug/mL)Dot Blot (1-2 ug/mL)Immunofluorescence (1:100)Flow Cytometry (1-2 ug/mL)Methylated DNA Immunoprecipitation (1-5 ug/IP)The optimal working dilution should be determined by the end user.
      • Storage Buffer:
      • In PBS
      • Storage Instruction:
      • Aliquot and store at -80°C.Aliquot to avoid repeated freezing and thawing.
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • Immunofluorescence
      • Immunofluorescence
      • Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952).A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um.B. Enlarged picture corresponding to a region from the left panel (as indicated in A).C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B).
      • Immunoprecipitation
      • Immunoprecipitation
      • MeDIP assay was performed using fragmented genomic DNA from the MCF7 breast cancer cells, 5-mC monoclonal antibody, clone b (Cat # MAB4952) and optimized PCR primer sets for qPCR of the indicated regions.1 ug of antibody was used per IP experiment.The hypermethylated p14 INK4A intronic CpG island and Alu repeats are clearly recovered, whereas the non-methylated promoter-CpG island of the p14 INK4A gene is not (red bars).One IP negative control (no antibody added) was included in the assay, the background is extremely low (white bars).
      • Enzyme-linked Immunoabsorbent Assay
      • Dot Blot
      • Methylated DNA Immunoprecipitation
      • Flow Cytometry
      • Application Image
      • Immunofluorescence
      • Immunofluorescence
      • enlarge
      • Immunoprecipitation
      • Immunoprecipitation
      • enlarge
      • Enzyme-linked Immunoabsorbent Assay
      • Dot Blot
      • Methylated DNA Immunoprecipitation
      • Flow Cytometry
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