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Millipore/MAB5272 | Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324/MAB5272/100 µg
  • Millipore/MAB5272 | Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324/MAB5272/100 µg

Millipore/MAB5272 | Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324/MAB5272/100 µg

價格: ¥4680.00 市場價: 7800.00

貨號: MAB5272
品牌: Millipore
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    • Description
      CatalogueNumberMAB5272
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-NeuralCellAdhesionMoleculeL1Antibody,clone324
      AlternateNames
      • CD171
      • N-CAML1
      BackgroundInformationFamiliesofadhesionmoleculeswhichsharecommoncarbohydratedomainsdoexist,despitethestructuralandfunctionaldiversityoftheseglycoproteins.TheseincludetheCa2+-independentneuraladhesionmolecules:N-CAM,myelinassociatedglycoprotein(MAG)andL1.L1isinvolvedinneuron-neuronadhesion,neuritefasciculation,outgrowthofneurites,cerebellargranulecellmigration,neuriteoutgrowthonSchwanncellsandinteractionsamongepithelialcellsofintestinalcrypts
      ProductInformation
      FormatPurified
      Control
      • Braintissue
      PresentationRatmonoclonalinbuffercontaining0.02MPhosphatebuffer,0.25MNaCl,pH7.6with0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8oCfromdateofreceipt.
      Applications
      ApplicationThisAnti-NeuralCellAdhesionMoleculeL1Antibody,clone324isvalidatedforuseinIC,IH,WBforthedetectionofNeuralCellAdhesionMoleculeL1.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesWesternblotting:
      20μg/mLwasusedonapreviouslot.

      Immunocytochemistry:
      5-10μg/mLwasusedonapreviouslot.Cellsmustbefixedwithmethanolfor10minutesat-20°Cinthemiddlelogphase.

      Immunohistochemistry:Clone324issensitivetofixation.Freshfrozen,acetoneor-20Cmethanolfixedtissuesarerecommended.TrADItional4%PFAtypicallydoesnotworkwell.Otherfixativeshavenotbeentested.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenRatswereimmunizedwithaglycoproteinfractionfromcerebellumof8-10dayoldC57BL/6Jmice.
      Clone324
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRat
      SpecificityMonoclonalantibodyagainstneuralcelladhesionmoleculeL1fromrat-rathybridcells.
      IsotypeIgG
      SpeciesReactivity
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisamemberoftheIgsuperfamilyandencodesacellsurfacesialoglycoproteinexpressedbycytokine-activatedendothelium.ThistypeImembraneproteinmediatesleukocyte-endothelialcelladhesionandsignaltransduction,andmayplayaroleinthedevelopmentofartherosclerosisandrheumatoidarthritis.Twoalternativelysplicedtranscriptsencodingdifferentisoformshavebeendescribedforthisgene.
      GeneSymbol
      • VCAM1
      • INCAM-100
      • MGC99561
      • CD106
      • DKFZp779G2333
      • L1CAM
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P19320#Importantincell-cellrecognition.Appearstofunctioninleukocyte-endothelialcelladhesion.Interactswiththebeta-1integrinVLA4onleukocytes,andmediatesbothadhesionandsignaltransduction.TheVCAM1/VLA4interactionmayplayapathophysiologicrolebothinimmuneresponsesandinleukocyteemigrationtositesofinflammation.
      SIZE:739aminoacids;81276Da
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Expressedoninflamedvascularendothelium,aswellasonmacrophage-likeanddendriticcelltypesinbothnormalandinflamedtissue.
      DOMAIN:SwissProt:P19320EitherthefirstorthefourthIg-likeC2-typedomainisrequiredforVLA4-dependentcelladhesion.
      PTM:Sialoglycoprotein.
      DISEASE:SwissProt:P19320#Mayplayanimportantroleinthegenesisofatherosclerosisandrheumatoidarthritis.
      SIMILARITY:Contains7Ig-likeC2-type(immunoglobulin-like)domains.
      MolecularWeight120-140kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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