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Acidphosphatases(AP)areafamilyofenzymesthatnon-specificallycatalyzethehydrolysisofmonoestersandanhydridesofphosphoricacidtoproduceinorganicphosphateatanoptimalpHof4-7.ChangesinthelevelsandactivitiesofAPoccurinmanypatho-physiologicalconditionsincludingprostratecancerandbonedisease.TheAPActivityFluorometricAssayKitprovidesasimpleanddirectprocedureformeasuringAPactivityinserumandothersamples.APactivityisdeterminedbytheconversionofthenon-fluorescent4-methyl-umbelliferylphosphatedisodiumsalt(MUP)tothefluorescentproduct4-methylumbelliferone(4-MU)(λex=360/λem=440nm),proportionaltotheAPactivitypresent.OneunitofAPistheamountofenzymethatwillhydrolyze1.0μmoleofMUPperminuteat25°C.
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