產(chǎn)品概述：產(chǎn)品參數(shù) 外觀：橙紅色液體 Abs (pH 3)= 464 nm (before photolysis) Abs/Em (after photocrosslinking to nucleic acid)= 510/610 nm 分子量：511.5 儲(chǔ)存條件 -20℃避光保存，有效期見(jiàn)外包裝。 產(chǎn)品介紹 PMA 是一種高親和性的 DNA 結(jié)合染料，該染料本身具有微弱的熒光，但與核酸結(jié)合后可以發(fā)出更為明亮的熒光。它尤其與雙鏈 DNA 具有高親和性。PMA 不能透過(guò)細(xì)胞膜，因此只能選擇性標(biāo)記死細(xì)胞上暴露在外的 DNA。這個(gè)特性使得 PMA 可以通過(guò)實(shí)時(shí)定量 PCR（qPCR）的手段，廣泛用于可被培養(yǎng)的病原細(xì)胞的篩選，因?yàn)?PMA 可與死細(xì)胞上的 DNA 牢固結(jié)合，而不能用于 PCR 反應(yīng)的擴(kuò)增。PMA 的這個(gè)特性使其可以通過(guò)實(shí)時(shí)定量 PCR 的手段，廣泛用于可被培養(yǎng)的病原細(xì)菌的篩選。注意事項(xiàng) 1. 熒光染料均存在淬滅問(wèn)題，請(qǐng)盡量注意避光，以減緩熒光淬滅。 2. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
說(shuō)明書(shū)：
UE-P4051 使用本產(chǎn)品的文獻(xiàn)：參考文獻(xiàn)1.Detection and quantification of viable Bacillus cereus group species in milk by propidium monoazide quantitative real-time PCR應(yīng)用方向：Bacillus cereus（蠟樣芽孢桿菌）2.Persistence of Bacteroides ovatus under simulated sunlight irradiation應(yīng)用方向：Bacteroides ovales（卵巢桿菌）3.Advantageous Direct Quantification of Viable Closely Related Probiotics inPetitSuisse Cheeses under In Vitro Gastrointestinal Conditions by Propidium Monoazide - qPCR應(yīng)用方向：Bifidobacterium animalis（動(dòng)物雙歧桿菌）4.Assessing the diagnostic importance of nonviable bacterial cells in respiratory infections應(yīng)用方向：Burkholderia cepacia（赤胸鯊）5.Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions應(yīng)用方向：Campylobacter jejuni（空腸彎曲菌）6.Viability-PCR Shows That NAAT Detects a High Proportion of DNA from Non-Viable Chlamydia trachomatis應(yīng)用方向：Chlamydia trachomatis（沙眼衣原體 ）7.Digestibility and prebiotic properties of potato rhamnogalacturonan I polysaccharide and its galactose-rich oligosaccharides/oligomers應(yīng)用方向：Clostridium leptum（瘦肉梭菌）8.Survival and persistence of host-associated Bacteroidales cells and DNA in comparison with Escherichia coli and Enterococcus in freshwater sediments as quantified by PMA-qPCR and qPCR應(yīng)用方向：Enterrococcus spp（腸球菌屬）9.Detection of viable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR應(yīng)用方向：Escherichia coli（大腸桿菌）10.Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR應(yīng)用方向：Helicobacter pylori（幽門(mén)螺桿菌）11.Probiotic bacteria survive in Cheddar cheese and modify populations of other lactic acid bacteria應(yīng)用方向：Lactobacillus acidophilus（嗜酸乳桿菌）12.Fate of Viable but Nonculturable Listeria monocytogenes in Pig Manure Microcosms應(yīng)用方向：Listeria monocytogenes（單球菌）13.A multicenter study of viable PCR using propidium monoazide to detect Legionella in water samples應(yīng)用方向：Legionella pneumophila（嗜肺軍團(tuán)菌）14.Propidium monoazide and Xpert MTB/RIF to quantify Mycobacterium tuberculosis cells應(yīng)用方向：Mycobacterium tuberculosis（結(jié)核分枝桿菌）15.Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods應(yīng)用方向：Nitrosomonas europae（歐洲硝酸銨）16.Study and Understanding Behavior of Alginate-Inulin Synbiotics Beads for Protection and Delivery of AntimicrobialProducing Probiotics in Colonic Simulated Conditions應(yīng)用方向：Pediococcus acidilactici（酸乳球菌）17.Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR應(yīng)用方向：Pseudomonas aeruginosa（銅綠假單胞菌）18.Multiplex PCR coupled with propidium monoazide for the detection of viable Cronobacter sakazakii, Bacillus cereus, and Salmonella spp. in milk and milk products應(yīng)用方向：Salmonella enterica（腸炎沙門(mén)菌）19.Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide-Modified Quantitative PCR應(yīng)用方向：Staphylococcus aureus（金黃色葡萄球菌）20.Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide應(yīng)用方向：Streptococcus oralis（口腔鏈球菌）21.OxyRactivated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis應(yīng)用方向：Vibrio cholerae（霍亂弧菌）22.A New Physiological Role for the DNA Molecule as a Protector against Drying Stress in Desiccation-Tolerant Microorganisms應(yīng)用方向：Microbacterium 3J1（微桿菌3J1)
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