Yeast FCY1 recombinant protein with His tag expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
77.5
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Concentration:
2 U/uL
Purity:
> 90% as determined by SDS-PAGE stained with Coomassie Blue
Activity:
1U FCY1 converts > 80% of 10 ug cytosine to uracil at 37°C in 90 minutes.
Storage Buffer:
In 20 mM Tris-HCl, 500 mM NaCl, pH8.0 (10% Glycerol)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Applications
Functional Study
SDS-PAGE
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Functional Study
SDS-PAGE
Gene Information
Entrez GeneID:
856175
GeneBank Accession#:
NM_001184159.1
Protein Accession#:
NP_015387.1
Gene Name:
FCY1
Gene Alias:
-
Gene Description:
Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
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