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Pierce/Qdot 605 Streptavidin Conjugate/50 µL/Q10103MP
  • Pierce/Qdot 605 Streptavidin Conjugate/50 µL/Q10103MP

Pierce/Qdot 605 Streptavidin Conjugate/50 µL/Q10103MP

價(jià)格: ¥2016.00 市場(chǎng)價(jià): 3360.00

貨號(hào): Q10103MP
品牌: Pierce
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    • Description

      Qdot®605streptavidinconjugatecomprisesabiotin-bindingprotein(streptavidin)covalentlyattachedtoafluorescentlabel(Qdot®nanocrystal).Streptavidinhasaveryhighbindingaffinityforbiotin,andaconjugateofstreptavidiniscommonlyusedtogetherwithaconjugateofbiotinforspecificdetectionofavarietyofproteins,proteinmotifs,nucleicacids,andothermolecules(forexample,abiotinylatedprimaryantibodyboundtoaproteintargetcanbedetectedwithafluorescentlylabeledstreptavidin).Strategiessimilartothisareusedinmanydetectionprotocolsincludingwesternblots,flowcytometry,imagingandmicroscopy,andmicroplateassays,andarealsousedinpurificationworkflowstoachievetargetedfractionation.Qdot®nanocrystalconjugatesareprovidedas1µMsolutions.

      ImportantFeaturesofQdot®StreptavidinConjugates:
      StreptavidinQdot®605conjugatehasemissionmaximaof~605nm
      Approximately5to10streptavidinsperQdot®nanocrystal
      Extremelyphotostableandbrightfluorescence
      Efficientlyexcitedwithsingle-lineexcitationsources
      Narrowemission,largeStokesshift
      Availableinmultiplecolors
      Idealforwesternblots,flowcytometry,imagingandmicroscopy,microplateassaysandmore

      PropertiesofQdot®Nanocrystals
      TheQdot®streptavidinconjugateisthesizeofalargemacromoleculeorprotein(~15–20nm)andrepresentsthebrightestclassofstreptavidindetectionreagents.Qdot®streptavidinconjugatesaremadefromananometer-scalecrystalofasemiconductormaterial(CdSe),whichiscoatedwithanadditionalsemiconductorshell(ZnS)toimprovetheopticalpropertiesofthematerial.TheQdot®705andQdot®800streptavidinconjugates,whichincludeCdSeTe,aremadeinasimilarfashion.Thiscore-shellmaterialisfurthercoatedwithapolymershellthatallowsthematerialstobeconjugatedtoBIOLOGicalmoleculesandtoretaintheiropticalproperties.

      OtherFluorescentConjugatesofStreptavidinareAvailable
      WeofferseveralotherQdot®colorsortrytheQdot®StreptavidinSamplerKit,containingQdot®streptavidinconjugatesinsixcolors525,565,585,605,655,and705.Inadditiontonanocrystalconjugates,weofferawiderangeofstreptavidinsconjugatedtoAlexaFluor®dyes,OregonGreen®dye,enzymeconjugates,andtrADItionalfluorophoreslikeTexasRed®dye,fluorescein(FITC)andmore.

      FindBiotinylatedConjugates
      Weofferanextensivelineofbiotinylatedconjugatesforuseinbiotin-streptavidindetectionstrategies.
      •Usetheprimaryantibodysearchtooltofindbiotinylatedprimaryantibodies
      •Usethesecondaryantibodyselectortooltofindbiotinylatedsecondaryantibodiesandbiotinylatedanti-dyeandanti-haptenantibodies

      BlockingEndogenousBiotin
      Naturallyoccurringbiotinscaninterferewithbiotin-streptavidindetectionschemes.ForexperimentsinvolvingfixedandpermeABIlizedcells,tryourEndogenousBiotin-BlockingKittominimizethisinterference.

      ForResearchUseOnly.Notintendedforanyanimalorhumantherapeuticordiagnosticuse.

      RelatedLinks:

      LearnmoreaboutAvidin-BiotinDetection

      TolearnmoreaboutQdot®Nanocrystals
      ForResearchUseOnly.Notforuseindiagnosticprocedures.

      Specifications

      LabelorDye:Qdot®605
      ProductLine:Qdot®
      ProductSize:200µL
      ShippingCondition:RoomTemperature

      Contents&storage

      Storeinrefrigerator(2–8°C).
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