?? High Fidelity (HF?) Restriction Enzymes have 100% activity in CutSmart? Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver? qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
NEB extensively performs quality controls on all standard and high-fidelity(HF?) restriction enzymes. Examples of nuclease contamination studies for someof our HF restriction enzymes are shown below.
Product Source
An?E. coli?strain that carries the cloned and modified SbfI gene from?Streptomycesspecies Bf-61 (S.K. Degtyarev).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart® Buffer
-20
10X
Gel Loading Dye, Purple (6X)
25
6X
Notes:
Based on the stability of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.