AFRICAN SWINE FEVER VIRUS P30 (E. COLI)

African Swine Fever virus p30 is a recombinant protein expressed and purified from E. coli cells. This protein is suitable for use in ELISA, Luminex and SDS-PAGE/Western blot.

PRODUCT DETAILS –? AFRICAN SWINE FEVER VIRUS P30 (E.COLI)

• Codon-optimised and Ubiquitin-fused recombinant p30, manufactured in E. coli with a His-tag.
• Protein purified by affinity chromatography and is greater than 90% pure by SDS-PAGE.
• Presented in phosphate buffered saline (PBS), pH 7.4, 8M urea.
• Suitable for use in ELISA, Western Blot, Luminex and SDS-PAGE. In ELISA and Luminex, the antigen detected anti-ASFV antibodies in serum and oral fluid of pigs inoculated with ASFV as well as pigs vaccinated with p30.

BACKGROUND

African swine fever virus (ASFV) is a large, double-stranded DNA virus and the sole member of the Asfarviridae family. ASFV infects domestic pigs, wild boars, warthogs, and bush pigs, as well as soft ticks (Ornithodoros erraticus), which likely act as a vector. The ASFV genome (170 to 193 kbp depending on isolate) encodes 150 to 200 proteins, of which ~50 of them are structural. These proteins include pp220, pp62, p72, p54, p30, and CD2v, and serve as the major component of virus particles with roles in attachment, entry, and replication (Jia et al., 2017). Outbreaks have been reported in Africa and parts of Europe, South America, and the Caribbean. More recently (since 2007) the disease has been reported in multiple countries across Africa, Asia and Europe, in both domestic and wild pigs (OIE, 2018). There is currently no approved vaccine for ASF.

P30 is a 30-kDa phosphoprotein of ASFV that is synthesized, membrane localized, and released into the culture medium at early times after infection. Sequence analysis of the p30 open reading frame predicts a highly antigenic protein with putative phosphorylation, glycosylation, and membrane attachment sites (Afonso et al., 1992). Among the structural proteins that compose the virion of ASFV, p30 is one of the most immunogenic proteins and being produced during early stage of ASFV infection makes it a good target for diagnostic assays to detect ASFV infection (Petrovan et al. 2019).

REFERENCES

• Afonso et al. (1992). Characterization of p30, a highly antigenic membrane and secreted protein of African swine fever virus. Virology. 189(1):368-73.
• Jia et al. (2017). Roles of African Swine Fever Virus Structural Proteins in Viral Infection. J Vet Res. 61(2):135-143.
• Petrovan et al. (2019). Development and characterization of monoclonal antibodies against p30 protein of African swine fever virus. Virus Res. 269:197632.