The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.View our videos on protocols, tips, and recycling Monarch.
Monarch Plasmid Miniprep Column DesignAdvantages:
Elute in as little as 30 μl
Prevent buffer retention and salt carry-over with optimized column design
Reduce hands on time with faster protocols and less spin time
Monitor completion of certain steps using colored buffer system
No need to add RNase before starting
Easily label columns using tab and frosted surfaces
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
No hazardous materials fees
Specifications
Culture Volume:
1-5 ml, not to exceed 15 OD units
Binding Capacity:
up to 20 μg
Plasmid Size:
up to 25 kb
Typical Recovery:
up to 20 μg. Yield depends on plasmid copy number, hoststrain, culture volume, and growth conditions.
Elution Volume:
≥ 30 μl
Purity:
A260/280 and A260/230 ≥ 1.8
Protocol Time:
10½ minutes of spin and incubation time
CompatibleDownstreamApplications:
restriction digestion and other enzymatic manipulations,transformation, transfection, DNA sequencing, PCR, labeling,cell-free protein synthesis, etc.
Monarch Plasmid Miniprep Kit ProtocolMonarch Plasmid Miniprep Kits consistently produce more concentrated plasmid DNA with equivalent yield, purity and functionality as compared to the leading supplierPreps were performed according to recommended protocols using 1.5 ml aliquots of the same overnight culture. One microliter of each prep was digested with HindIII-HF (NEB #R3104) to linearize the vector and the digests were resolved on a 1% w/v agarose gel.Plasmid DNA purified using the Monarch Plasmid Miniprep Kit produces transfection efficiencies equivalent to or better than plasmid DNA purified using the Qiagen QIAprep® Spin Miniprep KitPlasmid DNA encoding constitutively expressed GFP (pEGFP-C2) was prepared using either Monarch Plasmid Miniprep Kit or Qiagen QIAprep Spin Miniprep Kit. Four different cell lines (Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, in complex with 0.3 μl Lipofectamine 2000, and 10 μl Opti-MEM. Five replicates for each cell type were performed using both DNA preps. GFP expressing cells were counted by flow cytometry 48 hrs post-transfection with a minimum of 2000 events collected per well. Average percentage of cells expressing GFP from all replicates is graphed and used as a measure of transfection efficiency.DNA from Monarch Plasmid Miniprep Kit is reproduciblycompatible with DNA sequencing.Plasmid DNA from three separate preps was sequenced using BigDye® Terminator chemistry on an AppliedBiosystems 3730XL DNA Analyzer. The electropherograms demonstrate the quality of the DNA is reproducible.
This product is related to the following categories:
Plasmid Purification Products,
Nucleic Acid Purification Products
This product can be used in the following applications:
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