當(dāng)前位置 : NEB >>> NEB/Monarch? PCR & DNA Cleanup Kit (5 μg) | Purification Kits/250 preps/T1030L
NEB/Monarch? PCR & DNA Cleanup Kit (5 μg) | Purification Kits/250 preps/T1030L
  • NEB/Monarch? PCR & DNA Cleanup Kit (5 μg) | Purification Kits/250 preps/T1030L

NEB/Monarch? PCR & DNA Cleanup Kit (5 μg) | Purification Kits/250 preps/T1030L

價格: 試用 市場價: 0.00

貨號: T1030L
品牌: NEB
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品:無質(zhì)量問題不接受退換貨,下單前請仔細(xì)核對信息。
下單后請及時聯(lián)系客服核對商品價格,訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費(fèi)服務(wù)熱線

4000-520-616


  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. The protocol can also be modified to enable the purification of smaller DNA fragments, including oligonucleotides and ssDNA.View our videos on protocols, tips, and recycling Monarch.

      APPLICATIONS
      PCR cleanup DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc.
      Enzymatic reaction cleanupRestriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample.
      cDNA cleanupDNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides.
      Labeling cleanupUnincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate
      Plasmid cleanupPlasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit.
      Oligonucleotide cleanupssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥15 bp) can be purified using the Oligonucleotide Cleanup Protocol.
      Monarch DNA Cleanup Column DesignMonarch DNA Cleanup Column Design
      Monarch columns are designed for performanceMonarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
      Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
      Specifications
      DNA Sample Type:DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations).ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol.
      Binding Capacity:up to 5 μg
      DNA Size Range:~50 bp to 25 kbDNA≥ 15 bp to 25 kb (dsDNA) and DNA≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
      Typical Recovery:

      DNA (50 bp to 10 kb): 70–90% DNA (11–23 kb): 50–70%ssDNA≥ 18 nt and dsDNA≥ 15 bp: 70-85%

      Elution Volume:≥ 6 μl
      Purity:A260/280 > 1.8 and A260/230 > 1.8
      Protocol Time:5 minutes of spin and incubation time
      Compatible DownstreamApplications:ligation, restriction digestion, labeling and other enzymaticmanipulations, library construction and DNA sequencing.
      Monarch PCR & DNA Cleanup Kit (5 µg) ProtocolMonarch PCR & DNA Cleanup Kit (5 μg) Protocol
      Monarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the leading supplierMonarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the leading supplier. Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq? Quick-Load? 2X Master Mix (NEB #M0486). DNA was eluted in 20 μl (NEB) and 40 μl (Qiagen) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF? (NEB #R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material.
      Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq® Quick-Load® 2X Master Mix (NEB #M0486). DNA was eluted in 20 μl (NEB) and 40 μl (Qiagen) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF® (NEB #R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material.
      Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samplesMonarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II.
      Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II.
      This product is related to the following categories:
      DNA Cleanup Products,
      Nucleic Acid Purification Products
      This product can be used in the following applications:
      PCR & Reaction Cleanup,
      Nucleic Acid Purification
    售后保障
    螞蟻淘生物188,秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度,由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購、物流、銷售、售后,保障正品優(yōu)質(zhì)。以“快速好省,為科研提供好產(chǎn)品、好價格”為理念,直接鏈接原廠家,從全國各地原制造商嚴(yán)格挑選188款科研精品,剔除品牌溢價,188生物新電商,把好的產(chǎn)品帶給科研!? 力求給你最優(yōu)質(zhì)的商品。
  • Q:生物188產(chǎn)品正品保障嗎?
    A:生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn),在業(yè)界享有良好的口碑;自營商城,直接從廠家采購, 自己的團(tuán)隊(duì)負(fù)責(zé)國際物流和清關(guān),中間沒有第三方,所有流程嚴(yán)格把控,100%保證正品,假一罰十。

    Q:下單后可以修改訂單嗎?
    A:下單后的商品付款之前可以修改;訂單付款成功,需要聯(lián)系我們客服進(jìn)行修改;客服電話:4000-520-616

    Q:可以開發(fā)票嗎?
    A:本網(wǎng)站所售商品都是正規(guī)清關(guān),均開具16%正規(guī)發(fā)票,發(fā)票金額含配送費(fèi)金額,另有說明的除外。

    Q:商品幾天可以發(fā)貨?
    A:生物188商品,全部現(xiàn)貨銷售,付款后即可發(fā)貨,一般一周內(nèi)送達(dá)!

    Q:如何聯(lián)系商家?
    A:有任何問題夠可以電話咨詢我們,全國24小時免費(fèi)服務(wù)熱線:4000-520-616 或聯(lián)系我們的在線客服QQ:1570468124

    Q:收到的商品少了/發(fā)錯了怎么辦?
    A:同個訂單購買多個商品可能會分為一個以上包裹發(fā)出,可能不會同時送達(dá),建議查看訂單詳情是否是 部分發(fā)貨狀態(tài);如未收到,可聯(lián)系在線客服或者致電4000-520-616。

    Q:退換貨/維修需要多長時間?
    A:一般情況下,退貨處理周期為客戶收到產(chǎn)品一個月內(nèi)(以快遞公司顯示簽收時間為準(zhǔn)),包裝規(guī)格、 數(shù)量、品種不符,外觀毀損、短缺或缺陷,請?jiān)谑盏截?4小時內(nèi)申請退換貨;特殊商品以合同條款為準(zhǔn)。

何為188

極簡而嚴(yán)謹(jǐn),我們僅銷售188款生物醫(yī)學(xué)科研用品,款款都是爆款;因?yàn)樯偎跃劢?,聚焦甄選每一款產(chǎn)品,聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號

獲取最新優(yōu)惠活動通知
  • 品質(zhì)甄選,正品保證

  • 自營電商,廠家直采

  • 極簡主義,188精品