The Monarch RNA Cleanup Columns (10 µg) are a component of the Monarch RNA Cleanup Kit (10 µg) (NEB #T2030) and can be used to purify up to 10 µg of RNA from enzymatic reactions. The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 6 μl and is ready for use in a variety of downstream applications including RT-PCR, RNA library prep for NGS, and labelling.
Monarch RNA Cleanup Columns are also available for 50 µg (NEB #T2047) and 500 µg (NEB #T2057) binding capacities.
Figure 1: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 6 µl
rRNA (10 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #2030) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260 as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in as little as 6 µl.Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of <20 ng of RNA in as little as 6 µl
A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A260, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.
This product is related to the following categories:
RNA Cleanup Products,
RNA Reagents Products,
RNA Extraction & Purification Products,
Nucleic Acid Purification Products
This product can be used in the following applications:
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